Rabbit anti-Human CCL2 Polyclonal Antibody | anti-CCL2 antibody

CCL2, CT (C-C motif Chemokine 2, Small-inducible Cytokine A2, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic Protein 1, MCP-1, Monocyte Chemotactic And Activating Factor, MCAF, Monocyte Secretory Protein JE, HC11, SCYA2, MCP1) (Biotin)

Cat. Num. AAA6486057

• Gene Names: CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CCL2; Polyclonal Antibody; CT (C-C motif Chemokine 2; Small-inducible Cytokine A2; Monocyte Chemoattractant Protein 1; Monocyte Chemotactic Protein 1; MCP-1; Monocyte Chemotactic And Activating Factor; MCAF; Monocyte Secretory Protein JE; HC11; SCYA2; MCP1) (Biotin); anti-CCL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCL2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CCL2 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; ELISA: 1:1000; IF: 1:10-1:50; IHC: 1:50-1:100; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa68-98 from the C-terminal region of human CCL2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CCL2 Antibody (C-term) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with CCL2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Immunofluorescence analysis of CCL2 Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CCL2 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). )

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using CCL2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CCL2 antibody

Antibodies; Growth Factors; Cytokines

References

1. Saenz Lopez, P., Actas Urol Esp 33(5), 474-481 (2009). 2. Rollins, B.J., Genomics 10(2), 489-492 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4506841
• NCBI GeneID: 6347
• NCBI Accession #: NP_002973
• NCBI GenBank Nucleotide #: NM_002982.3
• UniProt Accession #: P13500; Q9UDF3; B2R4V3
• Molecular Weight: Predicted: 11 kDa; Observed: 14kDa
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: CCL2
• NCBI Official Synonym Symbols: HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• NCBI Protein Information: C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL2
• UniProt Synonym Gene Names: MCP1; SCYA2; MCAF; MCP-1
• UniProt Entry Name: CCL2_HUMAN

NCBI Description

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; rough endoplasmic reticulum; endocytic vesicle; perinuclear region of cytoplasm; dendrite; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; chemokine activity; CCR2 chemokine receptor binding; receptor binding; protein kinase activity

Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; cellular homeostasis; cell adhesion; neutrophil chemotaxis; organ regeneration; JAK-STAT cascade; response to amino acid stimulus; positive regulation of tumor necrosis factor production; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; response to ethanol; unfolded protein response, activation of signaling protein activity; response to bacterium; cellular response to insulin stimulus; response to mechanical stimulus; response to heat; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; signal transduction; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to wounding; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; humoral immune response; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To