Rabbit anti-Human, Mouse CCDC88C Polyclonal Antibody | anti-CCDC88C antibody

CCDC88C Antibody

Cat. Num. AAA9630377

• Gene Names: CCDC88C; DAPLE; HKRP2; SCA40; KIAA1509
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CCDC88C; Polyclonal Antibody; CCDC88C Antibody; ccdc88c; Coiled-coil domain-containing protein 88C; DAPLE; DAPLE_HUMAN; Dvl-associating protein with a high frequency of leucine residues; hDaple; HkRP2; Hook-related protein 2; KIAA1509; Protein Daple; anti-CCDC88C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CCDC88C Antibody detects endogenous levels of CCDC88C.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CCDC88C antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human CCDC88C.
• Conjugation: Unconjugated
• Similarity: The GBA (G-alpha binding and activating) motif mediates binding to the alpha subunits of guanine nucleotide-binding proteins (G proteins). The PDZ domain is required for localization to apical junctions. Belongs to the CCDC88 family.
• Subunit Structure: Homooligomer (By similarity). Interacts with DVL1 (via PDZ domain); dissociates following initiation of non-canonical Wnt signaling. Interacts (via C-terminus) with ligand-activated Wnt receptor FZD7; competes with DVL1 for binding to FZD7 and displaces DVL1 from ligand-activated FZD7. Interacts (via GBA motif) with guanine nucleotide-binding protein G(i) alpha subunits GNAI1, GNAI2 and GNAI3 (inactive GDP-bound form); interacts with higher affinity with GNAI1 and GNAI3 than with GNAI2 and interaction leads to G(i) alpha subunit activation. Does not interact with GNAO1.
• Function: Required for activation of guanine nucleotide-binding proteins (G-proteins) during non-canonical Wnt signaling. Binds to ligand-activated Wnt receptor FZD7, displacing DVL1 from the FZD7 receptor and leading to inhibition of canonical Wnt signaling. Acts as a non-receptor guanine nucleotide exchange factor by also binding to guanine nucleotide-binding protein G(i) alpha (Gi-alpha) subunits, leading to their activation. Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex, triggering non-canonical Wnt responses such as activation of RAC1 and PI3K-AKT signaling. Promotes apical constriction of cells via ARHGEF18.
• Note: Enriched at apical cell junctions.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CCDC88C antibody

Cytoplasm; Cell junction; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 148762940
• NCBI GeneID: 440193
• NCBI Accession #: NP_001073883.2
• NCBI GenBank Nucleotide #: NM_001080414.3
• UniProt Accession #: Q9P219; Q69YK1; Q7L1M2; Q86SX7; Q8IYG8
• Molecular Weight: 49,253 Da
• NCBI Official Full Name: protein Daple
• NCBI Official Synonym Full Names: coiled-coil domain containing 88C
• NCBI Official Symbol: CCDC88C
• NCBI Official Synonym Symbols: DAPLE; HKRP2; SCA40; KIAA1509
• NCBI Protein Information: protein Daple
• UniProt Protein Name: Protein Daple
• Protein Family: Protein
• UniProt Gene Name: CCDC88C
• UniProt Synonym Gene Names: hDaple; HkRP2

NCBI Description

This gene encodes a ubiquitously expressed coiled-coil domain-containing protein that interacts with the dishevelled protein and is a negative regulator of the Wnt signalling pathway. The protein encoded by this gene has a PDZ-domain binding motif in its C-terminus with which it interacts with the dishevelled protein. Dishevelled is a scaffold protein involved in the regulation of the Wnt signaling pathway. The Wnt signaling pathway plays an important role in embryonic development, tissue maintenance, and cancer progression. Mutations in this gene cause autosomal recessive, primary non-syndromic congenital hydrocephalus; a condition characterized by excessive accumulation of cerebrospinal fluid in the ventricles of the brain. [provided by RefSeq, Jan 2013]

Uniprot Description

Negative regulator of the canonical Wnt signaling pathway, acting downstream of DVL to inhibit CTNNB1/Beta-catenin stabilization (). May also activate the JNK signaling pathway (PubMed:25062847).