Rabbit anti-Human CCDC50 Polyclonal Antibody | anti-CCDC50 antibody

CCDC50, ID (CCDC50, C3orf6, Coiled-coil domain-containing protein 50, Protein Ymer) (Biotin)

Cat. Num. AAA6281763

• Gene Names: CCDC50; YMER; C3orf6; DFNA44
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCDC50; Polyclonal Antibody; ID (CCDC50; C3orf6; Coiled-coil domain-containing protein 50; Protein Ymer) (Biotin); Protein Ymer; anti-CCDC50 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CCDC50 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CCDC50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-114 amino acids from the Central region of human CCDC50.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CCDC50 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CCDC50 antibody detected the CCDC50 protein (arrow).)

Immunohistochemistry (IHC)

(CCDC50 Antibody (Center) (AP14241c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC50 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CCDC50 antibody

This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].

Product Categories/Family for anti-CCDC50 antibody

Antibodies; KIAA; orf

References

Farfsing, A., et al. Leukemia 23(11):2018-2026(2009). Kameda, H., et al. Biochem. Biophys. Res. Commun. 378(4):744-749(2009). Bohgaki, M., et al. Biochim. Biophys. Acta 1783(5):826-837(2008). Modamio-Hoybjor, S., et al. Am. J. Hum. Genet. 80(6):1076-1089(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 28372509
• NCBI GeneID: 152137
• NCBI Accession #: NP_777568.1; NP_848018.1
• NCBI GenBank Nucleotide #: NM_174908.3
• UniProt Accession #: Q8IVM0; Q86VH7
• Molecular Weight: 35,822 Da
• NCBI Official Full Name: coiled-coil domain-containing protein 50 short isoform
• NCBI Official Synonym Full Names: coiled-coil domain containing 50
• NCBI Official Symbol: CCDC50
• NCBI Official Synonym Symbols: YMER; C3orf6; DFNA44
• NCBI Protein Information: coiled-coil domain-containing protein 50; protein Ymer
• UniProt Protein Name: Coiled-coil domain-containing protein 50
• Protein Family: Coiled-coil domain-containing protein
• UniProt Gene Name: CCDC50
• UniProt Synonym Gene Names: C3orf6
• UniProt Entry Name: CCD50_HUMAN

Uniprot Description

CCDC50 iso2: phosphorylated in response to EGFR signaling in human mammary epithelial cells. Two alternatively-spliced isoforms have been described.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: cytoplasm

Molecular Function: protein binding; ubiquitin protein ligase binding

Disease: Deafness, Autosomal Dominant 44