Western Blot (WB)
(CCDC36 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CCDC36 antibody detected the CCDC36 protein (arrow).)
Rabbit anti-Human CCDC36 Polyclonal Antibody | anti-CCDC36 antibody
CCDC36, CT (CCDC36, Coiled-coil domain-containing protein 36, Cancer/testis antigen 74) (Azide free) (HRP)
Gene Names
CCDC36; CT74
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CCDC36; Polyclonal Antibody; CT (CCDC36; Coiled-coil domain-containing protein 36; Cancer/testis antigen 74) (Azide free) (HRP); Cancer/testis antigen 74; anti-CCDC36 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-CCDC36 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CCDC36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-448 amino acids from the C-terminal region of human CCDC36.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(CCDC36 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CCDC36 antibody detected the CCDC36 protein (arrow).)
Western Blot (WB)
(CCDC36 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CCDC36 antibody detected the CCDC36 protein (arrow).)
Immunohistochemistry (IHC)
(CCDC36 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC36 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CCDC36 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC36 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CCDC36 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CCDC36 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-CCDC36 antibody
References
Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,343 Da
NCBI Official Full Name
coiled-coil domain-containing protein 36
NCBI Official Synonym Full Names
coiled-coil domain containing 36
NCBI Official Symbol
CCDC36
NCBI Official Synonym Symbols
CT74
NCBI Protein Information
coiled-coil domain-containing protein 36; cancer/testis antigen 74
UniProt Protein Name
Coiled-coil domain-containing protein 36
Protein Family
UniProt Gene Name
CCDC36
UniProt Synonym Gene Names
CT74
UniProt Entry Name
CCD36_HUMAN
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Product Notes
The CCDC36 ccdc36 (Catalog #AAA6281699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCDC36, CT (CCDC36, Coiled-coil domain-containing protein 36, Cancer/testis antigen 74) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC36 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CCDC36 ccdc36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCDC36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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