Western Blot (WB)
(Western blot analysis of lysates from 293,Neuro-2a cell line and mouse liver tissue (from left to right),using CCDC36 Antibody (C-term). MBS9211980 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Rabbit anti-Human CCDC36 Polyclonal Antibody | anti-CCDC36 antibody
CCDC36 Antibody (C-term)
Gene Names
CCDC36; CT74
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CCDC36; Polyclonal Antibody; CCDC36 Antibody (C-term); Coiled-coil domain-containing protein 36; Cancer/testis antigen 74; CT74; anti-CCDC36 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CCDC36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 422-450 amino acids from the C-terminal region of human CCDC36.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
422-450
Sequence Length
594
Applicable Applications for anti-CCDC36 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from 293,Neuro-2a cell line and mouse liver tissue (from left to right),using CCDC36 Antibody (C-term). MBS9211980 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from 293,Neuro-2a cell line and mouse liver tissue (from left to right),using CCDC36 Antibody (C-term). MBS9211980 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Immunohistochemistry (IHC)
(CCDC36 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC36 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CCDC36 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC36 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CCDC36 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CCDC36 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-CCDC36 antibody
References
Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66343
NCBI Official Full Name
coiled-coil domain-containing protein 36
NCBI Official Synonym Full Names
coiled-coil domain containing 36
NCBI Official Symbol
CCDC36
NCBI Official Synonym Symbols
CT74
NCBI Protein Information
coiled-coil domain-containing protein 36
UniProt Protein Name
Coiled-coil domain-containing protein 36
Protein Family
UniProt Gene Name
CCDC36
UniProt Synonym Gene Names
CT74
UniProt Entry Name
CCD36_HUMAN
Uniprot Description
CCDC36: 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 3p21.31
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Product Notes
The CCDC36 ccdc36 (Catalog #AAA9211980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 422-450. The CCDC36 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC36 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the CCDC36 ccdc36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCDC36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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