Western Blot (WB)
(HEK293 overexpressing Human CCDC3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with (0.5ug/ml) in the right panel (empty vector transfection in first lanes). Data obtained from Dr. YangXin Fu, Dept Oncology, University of Alberta, Edmonton, Canada.)
Goat CCDC3 Polyclonal Antibody | anti-CCDC3 antibody
Goat anti-CCDC3 (aa134-148) Antibody
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CCDC3; Polyclonal Antibody; Goat anti-CCDC3 (aa134-148) Antibody; coiled-coil domain containing 3; coiled-coil domain-containing protein 3; fat/vessel-derived secretory protein; favine; CCDC3 antibody; coiled-coil domain containing 3 antibody; coiled-coil domain-containing protein 3 antibody; fat/vessel-derived secretory protein antibody; favine antibody; CCDC3 (aa134-148); anti-CCDC3 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PHGVNFQDAIFPDTQ
Sequence Length
270
Applicable Applications for anti-CCDC3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: In transfected HEK293 transiently expressing Human CCDC3 (DYKDDDDK tagged), a band of approx.36kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-DYKDDDDK antibody. Recommended concentration, 0.5-1ug/ml.
Western Blot: In transfected HEK293 transiently expressing Human CCDC3 (DYKDDDDK tagged), a band of approx.36kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-DYKDDDDK antibody. Recommended concentration, 0.5-1ug/ml.
Immunogen
Peptide with sequence C-PHGVNFQDAIFPDTQ, from the internal region of the protein sequence according to NP_113643.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(HEK293 overexpressing Human CCDC3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with (0.5ug/ml) in the right panel (empty vector transfection in first lanes). Data obtained from Dr. YangXin Fu, Dept Oncology, University of Alberta, Edmonton, Canada.)
Western Blot (WB)
(HEK293 overexpressing Human CCDC3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with (0.5ug/ml) in the right panel (empty vector transfection in first lanes). Data obtained from Dr. YangXin Fu, Dept Oncology, University of Alberta, Edmonton, Canada.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,864 Da
NCBI Official Full Name
coiled-coil domain-containing protein 3 isoform 1
NCBI Official Synonym Full Names
coiled-coil domain containing 3
NCBI Official Symbol
CCDC3
NCBI Protein Information
coiled-coil domain-containing protein 3
UniProt Protein Name
Coiled-coil domain-containing protein 3
Protein Family
UniProt Gene Name
CCDC3
UniProt Synonym Gene Names
Favine
UniProt Entry Name
CCDC3_HUMAN
Uniprot Description
CCDC3: Up-regulated by pioglitazone and down-regulated by TNF- alpha in HUVEC. Homodimer.
Protein type: Secreted; Endoplasmic reticulum; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: endoplasmic reticulum; extracellular region
Research Articles on CCDC3
Similar Products
Product Notes
The CCDC3 ccdc3 (Catalog #AAA423049) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CCDC3 (aa134-148) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: In transfected HEK293 transiently expressing Human CCDC3 (DYKDDDDK tagged), a band of approx.36kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-DYKDDDDK antibody. Recommended concentration, 0.5-1ug/ml. Researchers should empirically determine the suitability of the CCDC3 ccdc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PHGVNFQDAI FPDTQ. It is sometimes possible for the material contained within the vial of "CCDC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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