Rabbit anti-Human, Mouse CBX4 Polyclonal Antibody | anti-CBX4 antibody

CBX4, NT (E3 SUMO-protein Ligase CBX4, Chromobox Protein Homolog 4, Polycomb 2 Homolog, Pc2, hPc2) (AP)

Cat. Num. AAA6467796

• Gene Names: CBX4; PC2; NBP16
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CBX4; Polyclonal Antibody; NT (E3 SUMO-protein Ligase CBX4; Chromobox Protein Homolog 4; Polycomb 2 Homolog; Pc2; hPc2) (AP); anti-CBX4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CBX4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CBX4 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:1000; IHC-P: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 80-110 in the N-terminal region of human CBX4, UniProt Accession #O00257.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot using MBS627364 to detect CBX4 in mouse kidney tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS627364 which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.)

Product Categories/Family for anti-CBX4 antibody

Antibodies; Chromatin Proteins

References

1. Kagey, M.H., et al., Cell 113(1):127-137 (2003). 2. Satijn, D.P., et al., Mol. Cell. Biol. 17(10):6076-6086 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 55770830
• NCBI GeneID: 8535
• NCBI Accession #: NP_003646
• NCBI GenBank Nucleotide #: NM_003655.2
• UniProt Accession #: O00257; Q6TPI8; Q96C04; B1PJR7
• Molecular Weight: 62 kDa
• NCBI Official Full Name: E3 SUMO-protein ligase CBX4
• NCBI Official Synonym Full Names: chromobox homolog 4
• NCBI Official Symbol: CBX4
• NCBI Official Synonym Symbols: PC2; NBP16
• NCBI Protein Information: E3 SUMO-protein ligase CBX4; Pc class 2 homolog; polycomb 2 homolog; NS5ATP1-binding protein 16; chromobox protein homolog 4; chromobox homolog 4 (Pc class homolog, Drosophila)
• UniProt Protein Name: E3 SUMO-protein ligase CBX4
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: CBX4
• UniProt Synonym Gene Names: Pc2; hPc2
• UniProt Entry Name: CBX4_HUMAN

Uniprot Description

CBX4: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; SUMO ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; nuclear speck; PcG protein complex; nucleus

Molecular Function: protein binding; enzyme binding; single-stranded RNA binding; SUMO binding; phosphoprotein binding; chromatin binding; transcription corepressor activity; methylated histone residue binding; ligase activity

Biological Process: protein sumoylation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis