Western Blot (WB)
((0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CBR3 Polyclonal Antibody | anti-CBR3 antibody
Goat anti-CBR3 Antibody
Gene Names
CBR3; hCBR3; SDR21C2; HEL-S-25
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CBR3; Polyclonal Antibody; Goat anti-CBR3 Antibody; carbonyl reductase 3; carbonyl reductase (NADPH) 3; SDR21C2; hCBR3; NADPH-dependent carbonyl reductase 3; short chain dehydrogenase/reductase family 21C; member 2; CBR3 antibody; carbonyl reductase 3 antibody; carbonyl reductase (NADPH) 3 antibody; SDR21C2 antibody; hCBR3 antibody; NADPH-dependent carbonyl reductase 3 antibody; member 2 antibody; anti-CBR3 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QGQLVHDKVVQNW
Sequence Length
277
Applicable Applications for anti-CBR3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 30.9kDa according to NP_001227.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bains et al, J Pharmacol Exp Ther.2010 Mar;332(3): 755-63.; PMID: 20007405). Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human recombinant protein.
Western Blot: Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 30.9kDa according to NP_001227.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bains et al, J Pharmacol Exp Ther.2010 Mar;332(3): 755-63.; PMID: 20007405). Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human recombinant protein.
Immunogen
Peptide with sequence C-QGQLVHDKVVQNW, from the C Terminus of the protein sequence according to NP_001227.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,850 Da
NCBI Official Full Name
carbonyl reductase
NCBI Official Synonym Full Names
carbonyl reductase 3
NCBI Official Symbol
CBR3
NCBI Official Synonym Symbols
hCBR3; SDR21C2; HEL-S-25
NCBI Protein Information
carbonyl reductase [NADPH] 3
UniProt Protein Name
Carbonyl reductase [NADPH] 3
Protein Family
UniProt Gene Name
CBR3
UniProt Entry Name
CBR3_HUMAN
NCBI Description
Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008]
Uniprot Description
CBR3: Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: EC 1.1.1.184; Lipid Metabolism - arachidonic acid; Oxidoreductase
Chromosomal Location of Human Ortholog: 21q22.2
Cellular Component: cytoplasm; cytosol; extracellular space; nucleoplasm
Molecular Function: 3-keto sterol reductase activity; carbonyl reductase (NADPH) activity
Biological Process: cognition; phylloquinone catabolic process
Research Articles on CBR3
Similar Products
Product Notes
The CBR3 cbr3 (Catalog #AAA420447) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CBR3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CBR3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 30.9kDa according to NP_001227.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bains et al, J Pharmacol Exp Ther.2010 Mar;332(3): 755-63.; PMID: 20007405). Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human recombinant protein. Researchers should empirically determine the suitability of the CBR3 cbr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QGQLVHDKVV QNW. It is sometimes possible for the material contained within the vial of "CBR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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