Rabbit anti-Human CBFA2T2 Polyclonal Antibody | anti-CBFA2T2 antibody

CBFA2T2 Antibody

Cat. Num. AAA7044438

• Gene Names: CBFA2T2; EHT; p85; MTGR1; ZMYND3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: CBFA2T2; Polyclonal Antibody; CBFA2T2 Antibody; Protein CBFA2T2; ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; Myeloid translocation-related protein 1; p85; EHT; MTGR1; anti-CBFA2T2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 595

• Applicable Applications for anti-CBFA2T2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Protein CBFA2T2 protein (C-280AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-55966 / sc-55967
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: CBFA2T2 antibody at 1.82ug/mlLane 1: 293T whole cell lysateLane 2: Raji whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 68,64,29,66 kDaObserved band size: 67 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7044438 at dilution 1:100)

Related Product Information for anti-CBFA2T2 antibody

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region.

NCBI and Uniprot Product Information

• NCBI GI #: 74229008
• NCBI GeneID: 9139
• NCBI Accession #: NP_001028171.1
• NCBI GenBank Nucleotide #: NM_001032999.2
• UniProt Accession #: O43439; Q5TGE4; Q5TGE5; Q5TGE6; Q5TGE7; Q8IWF3; Q96B06; Q96L00; Q9H436; Q9UJP8; B2RAE6; F8W6D7
• Molecular Weight: 65,878 Da
• NCBI Official Full Name: protein CBFA2T2 isoform MTGR1c
• NCBI Official Synonym Full Names: CBFA2/RUNX1 translocation partner 2
• NCBI Official Symbol: CBFA2T2
• NCBI Official Synonym Symbols: EHT; p85; MTGR1; ZMYND3
• NCBI Protein Information: protein CBFA2T2
• UniProt Protein Name: Protein CBFA2T2
• Protein Family: Protein
• UniProt Gene Name: CBFA2T2
• UniProt Synonym Gene Names: EHT; MTGR1
• UniProt Entry Name: MTG8R_HUMAN

NCBI Description

In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency (PubMed:27281218). Involved in primordial germ cell (PCG) formation. Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent manner (). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 (AML1-MTG8/ETO fusion protein) which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (). Required for the maintenance of the secretory cell lineage in the small intestine. Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation ().