Rabbit anti-Human Caveolin-2 Polyclonal Antibody | anti-Y19 antibody

Caveolin-2 (Phospho-Y19) Polyclonal Antibody

Cat. Num. AAA3007916

• Gene Names: CAV2; CAV
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Caveolin-2; Polyclonal Antibody; Caveolin-2 (Phospho-Y19) Polyclonal Antibody; Caveolin2; CAV2; Caveolin 2; anti-Y19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caveolin-2 (phospho-Y19) polyclonal antibody detects endogenous levels of Caveolin-2 protein only when phosphorylated at Tyr19.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 149

• Applicable Applications for anti-Y19 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Caveolin-2 around the phosphorylation site of Tyrosine19.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Caveolin-2 (phospho-Y19) polyclonal antibody in paraffin-embedded human prostate tissue at 1:50.)

Related Product Information for anti-Y19 antibody

The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids. Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease. Caveolins interact with multiple signaling molecules such as Galpha subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS. It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins such as GRB7. Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 332164664
• NCBI GeneID: 858
• NCBI Accession #: NP_001193676.1
• NCBI GenBank Nucleotide #: NP_001193676.1
• UniProt Accession #: P51636
• Molecular Weight: ~ 18kDa
• NCBI Official Full Name: caveolin-2 isoform b
• NCBI Official Synonym Full Names: caveolin 2
• NCBI Official Symbol: CAV2
• NCBI Official Synonym Symbols: CAV
• NCBI Protein Information: caveolin-2
• UniProt Protein Name: Caveolin-2
• Protein Family: Caveolin
• UniProt Gene Name: CAV2
• UniProt Entry Name: CAV2_HUMAN

NCBI Description

The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. This gene and related family member (CAV1) are located next to each other on chromosome 7, and express colocalizing proteins that form a stable hetero-oligomeric complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Additional isoforms resulting from the use of alternate in-frame translation initiation codons have also been described, and shown to have preferential localization in the cell (PMID:11238462). [provided by RefSeq, May 2011]

Uniprot Description

Caveolin-2: a scaffolding protein. A component of caveolae, small invaginations of the plasma membrane found in most cell types. Involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Caveolin-2 may function as an accessory protein in conjunction with caveolin-1. Two splice-variant isoforms have been observed.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q31.1

Cellular Component: Golgi apparatus; focal adhesion; protein complex; integral to plasma membrane; caveola; acrosomal membrane; lipid raft; Golgi membrane; transport vesicle; extrinsic to internal side of plasma membrane; membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; intracellular; nucleus

Molecular Function: protein binding; protein homodimerization activity; D1 dopamine receptor binding

Biological Process: endoplasmic reticulum organization and biogenesis; positive regulation of dopamine receptor signaling pathway; mitochondrion organization and biogenesis; vesicle organization and biogenesis; skeletal muscle fiber development; protein oligomerization; vesicle fusion; vesicle docking; positive regulation of MAPKKK cascade; negative regulation of endothelial cell proliferation; insulin receptor signaling pathway; regulation of mitosis; positive regulation of endothelial cell proliferation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of GTPase activity