Rabbit CathepsinF Polyclonal Antibody | anti-CTSF antibody

CathepsinF Antibody

Cat. Num. AAA9611630

• Gene Names: CTSF; CATSF; CLN13
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CathepsinF; Polyclonal Antibody; CathepsinF Antibody; AI481912; CATF_HUMAN; Cathepsin F; CATSF; CLN13; Ctsf; EC 3.4.22.41; anti-CTSF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CathepsinF Antibody detects endogenous levels of CathepsinF.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 484

• Applicable Applications for anti-CTSF antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human CathepsinF.
• Tissue Specificity: High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Lysosome
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse liver tissue?, using CathepsinF Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain, using CathepsinF Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-CTSF antibody

Description: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Similarity: Belongs to the peptidase C1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 6042196
• NCBI GeneID: 8722
• NCBI Accession #: NP_003784.2
• NCBI GenBank Nucleotide #: NP_003784.2
• UniProt Accession #: Q9UBX1
• Molecular Weight: Observed: 53 kDa; Predicted: 54 kDa
• NCBI Official Full Name: cathepsin F
• NCBI Official Synonym Full Names: cathepsin F
• NCBI Official Symbol: CTSF
• NCBI Official Synonym Symbols: CATSF; CLN13
• NCBI Protein Information: cathepsin F
• UniProt Protein Name: Cathepsin F
• Protein Family: Cathepsin
• UniProt Gene Name: CTSF
• UniProt Synonym Gene Names: CATSF
• UniProt Entry Name: CATF_HUMAN

NCBI Description

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSF: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.22.41

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: lysosomal lumen; lysosome; vesicle

Molecular Function: cysteine-type endopeptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 13