Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Catalase (CAT) Polyclonal Antibody | anti-CAT antibody
Polyclonal Antibody to Catalase (CAT)
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRL ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA ARE
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: CAT knockout HepG2 cell lysate; Predicted MW: 60kd Observed MW: 60kd Primary Ab: 2ug/ml Rabbit Anti-Human CAT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: CAT knockout HepG2 cell lysate; Predicted MW: 60kd Observed MW: 60kd Primary Ab: 2ug/ml Rabbit Anti-Human CAT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.
Protein type: Amino Acid Metabolism - tryptophan; Apoptosis; EC 1.11.1.6; Endoplasmic reticulum; Energy Metabolism - methane; Hydrolase; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: cytosol; endoplasmic reticulum; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; focal adhesion; Golgi apparatus; intracellular membrane-bound organelle; lysosome; membrane; mitochondrial intermembrane space; mitochondrion; peroxisomal matrix; peroxisomal membrane; peroxisome; plasma membrane; secretory granule lumen
Molecular Function: aminoacylase activity; antioxidant activity; catalase activity; enzyme binding; heme binding; metal ion binding; NADP binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; receptor binding
Biological Process: aerobic respiration; aging; cellular oxidant detoxification; cellular response to growth factor stimulus; cellular response to oxidative stress; cholesterol metabolic process; hemoglobin metabolic process; hydrogen peroxide catabolic process; negative regulation of apoptosis; negative regulation of NF-kappaB transcription factor activity; neutrophil degranulation; osteoblast differentiation; positive regulation of cell division; positive regulation of NF-kappaB transcription factor activity; positive regulation of phosphoinositide 3-kinase cascade; protein homotetramerization; protein tetramerization; response to activity; response to cadmium ion; response to drug; response to estradiol; response to ethanol; response to hydrogen peroxide; response to hyperoxia; response to hypoxia; response to inactivity; response to insulin stimulus; response to L-ascorbic acid; response to lead ion; response to light intensity; response to ozone; response to reactive oxygen species; response to vitamin A; response to vitamin E; triacylglycerol metabolic process; ureteric bud development; UV protection
Disease: Acatalasemia
Research Articles on CAT
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Product Notes
The CAT cat (Catalog #AAA2003838) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Catalase (CAT) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Catalase (CAT) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CAT cat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRL ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA ARE. It is sometimes possible for the material contained within the vial of "Catalase (CAT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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