Rabbit CASZ1 Polyclonal Antibody | anti-CASZ1 antibody

Rabbit Polyclonal to Human CASZ1

Cat. Num. AAA242378

• Gene Names: CASZ1; CST; SRG; CAS11; ZNF693; dJ734G22.1
• Reactivity: Bovine, Chimpanzee, Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CASZ1; Polyclonal Antibody; Rabbit Polyclonal to Human CASZ1; Anti-CASZ1 Antibody (N-Terminus) IHC-plus; Castor-related protein; CAS11; Castor; Castor homolog 1; zinc finger; CST; DJ734G22.1; Zinc finger protein 693; SRG; ZNF693; Castor zinc finger 1; Survival-related; Human CASZ1; anti-CASZ1 antibody

• Host: Rabbit
• Reactivity: Bovine, Chimpanzee, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: human Cas5 protein. A BLAST analysis was used to suggest cross reactivity with Cas5 from mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with Cas5 from other sources has not been determined.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1166

• Applicable Applications for anti-CASZ1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:25000 - 1:100000), IHC-P (5 ug/ml), WB (1:1000 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein according to accession number CAI22571.
• Immunogen Type: Synthetic peptide
• Immunogen: CASZ1 antibody was raised against synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein according to accession number CAI22571.
• Antigen Modification: N-Terminus
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CASZ1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-hCas5 Antibody - Western Blot. Western blot of affinity purified anti-hCas5 antibody shows detection of a band ~125 kD in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kD). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.)

NCBI and Uniprot Product Information

• NCBI GI #: 119709822
• NCBI GeneID: 54897
• NCBI Accession #: NP_060236.3
• NCBI GenBank Nucleotide #: NM_017766.4
• UniProt Accession #: Q86V15; Q078S9; Q2EN02; Q5T9S1; Q6ZNM8; Q8WX49; Q8WX50; Q9BT16; Q9NXC6
• Molecular Weight: 124,788 Da
• NCBI Official Full Name: zinc finger protein castor homolog 1 isoform b
• NCBI Official Synonym Full Names: castor zinc finger 1
• NCBI Official Symbol: CASZ1
• NCBI Official Synonym Symbols: CST; SRG; CAS11; ZNF693; dJ734G22.1
• NCBI Protein Information: zinc finger protein castor homolog 1; survival-related; castor-related protein; zinc finger protein 693; castor homolog 1, zinc finger
• UniProt Protein Name: Zinc finger protein castor homolog 1
• Protein Family: Zinc finger protein castor
• UniProt Gene Name: CASZ1
• UniProt Synonym Gene Names: CST; SRG; ZNF693
• UniProt Entry Name: CASZ1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor. The encoded protein may function as a tumor suppressor, and single nucleotide polymorphisms in this gene are associated with blood pressure variation. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

CASZ1: Probable transcription factor. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm

Molecular Function: DNA binding; metal ion binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; multicellular organismal development