Rabbit anti-Human Caspase 7 (CASP7) Polyclonal Antibody | anti-CASP7 antibody

Polyclonal Antibody to Caspase 7 (CASP7)

Cat. Num. AAA2004030

• Gene Names: CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Caspase 7 (CASP7); Polyclonal Antibody; Polyclonal Antibody to Caspase 7 (CASP7); anti-CASP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against CASP7. It has been selected for its ability to recognize CASP7 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ANPR YKIPVEADFL FAYSTVPGYY SWRSPGRGSW FVQALCSILE EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP
• Sequence Length: 407

• Applicable Applications for anti-CASP7 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant CASP7 (Ala207~Gln303) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2042528
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant CASP7, Human.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 4502581
• NCBI GeneID: 840
• NCBI Accession #: NP_001218.1
• NCBI GenBank Nucleotide #: NM_001227.4
• UniProt Accession #: P55210; Q13364; Q53YD5; Q5SVL0; Q5SVL3; Q96BA0; B4DQU7; B5BU45; D3DRB8
• Molecular Weight: 31,614 Da
• NCBI Official Full Name: caspase-7 isoform alpha
• NCBI Official Synonym Full Names: caspase 7
• NCBI Official Symbol: CASP7
• NCBI Official Synonym Symbols: MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• NCBI Protein Information: caspase-7
• UniProt Protein Name: Caspase-7
• Protein Family: Caspase
• UniProt Gene Name: CASP7
• UniProt Synonym Gene Names: MCH3; CASP-7; ICE-LAP3

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.