Immunohistochemistry (IHC)
(Fig.1. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Rabbit Caspase-3 Polyclonal Antibody | anti-CASP3 antibody
Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody
IF: 1:50-1:300
IHC-P: 1:50-1:300
ELISA: 1:20000
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Fig.1. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.1. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieva l (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieva l (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunofluorescence (IF)
(Fig.4. Immunofluorescence analysis of human lung cancer tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody (red) was diluted at 1:200 (4 degree C,overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.4. Immunofluorescence analysis of human lung cancer tissue. 1, Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody (red) was diluted at 1:200 (4 degree C,overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Western Blot (WB)
(Fig.5. Western Blot analysis of Hela (1), mouse samples (2), rat heart (3), yeast protein (4), rat lung (5), rat brain (6), mouse colon (7), mouse kidney (8), diluted at 1:5000.)
Western Blot (WB)
(Fig.5. Western Blot analysis of Hela (1), mouse samples (2), rat heart (3), yeast protein (4), rat lung (5), rat brain (6), mouse colon (7), mouse kidney (8), diluted at 1:5000.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
Uniprot Description
CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.
Protein type: Protease; EC 3.4.22.56; Apoptosis; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q34
Cellular Component: nucleoplasm; plasma membrane; nucleus; cytosol
Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity
Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; proteolysis; regulation of caspase activity; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; response to UV; cell structure disassembly during apoptosis; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; caspase activation via cytochrome c; platelet formation; response to DNA damage stimulus; induction of apoptosis by oxidative stress; negative regulation of apoptosis
Research Articles on CASP3
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Product Notes
The CASP3 casp3 (Catalog #AAA9700614) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IF: 1:50-1:300 IHC-P: 1:50-1:300 ELISA: 1:20000. Researchers should empirically determine the suitability of the CASP3 casp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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