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Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse CASP12 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Caspase 12 Polyclonal Antibody | anti-CASP12 antibody
Polyclonal Antibody to Caspase 12 (CASP12)
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Caspase 12; Polyclonal Antibody; Polyclonal Antibody to Caspase 12 (CASP12); anti-CASP12 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CASP12. It has beenselected for its ability to recognize CASP12 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MAARRTHERD PIYKIKGLAK DMLDGVFDDL VEKNVLNGDE LLKIGESASF ILNKAENLVE NFLEKTDMAG KIFAGHIANS QEQLSLQFSN DEDDGPQKIC TPSSPSESKR KVEDDEMEVN AGLAHESHLM LTAPHGLQSS EVQDTLKLCP RDQFCKIKTE RAKEIYPVME KEGRTRLALI ICNKKFDYLF DRDNADTDIL NMQELLENLG YSVVLKENLT AQEMETELMQ FAGRPEHQSS DSTFLVFMSH
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MAARRTHERD PIYKIKGLAK DMLDGVFDDL VEKNVLNGDE LLKIGESASF ILNKAENLVE NFLEKTDMAG KIFAGHIANS QEQLSLQFSN DEDDGPQKIC TPSSPSESKR KVEDDEMEVN AGLAHESHLM LTAPHGLQSS EVQDTLKLCP RDQFCKIKTE RAKEIYPVME KEGRTRLALI ICNKKFDYLF DRDNADTDIL NMQELLENLG YSVVLKENLT AQEMETELMQ FAGRPEHQSS DSTFLVFMSH
Sequence Length
420
Applicable Applications for anti-CASP12 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CASP12 (Met1~Phe413)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant CASP12 (Met1~Phe413) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2045945
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse CASP12 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse CASP12 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,854 Da
NCBI Official Full Name
caspase-12
NCBI Official Synonym Full Names
caspase 12
NCBI Official Symbol
Casp12
NCBI Protein Information
caspase-12
UniProt Protein Name
Caspase-12
Protein Family
UniProt Gene Name
Casp12
UniProt Synonym Gene Names
CASP-12
Uniprot Description
CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.22.-; Protease
Chromosomal Location of Human Ortholog: 9 A1|9 2.46 cM
Cellular Component: cytosol; endoplasmic reticulum; intracellular membrane-bound organelle; nucleus
Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; hydrolase activity; peptidase activity; protease binding; protein binding
Biological Process: apoptosis; endoplasmic reticulum unfolded protein response; positive regulation of apoptosis; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; protein autoprocessing; proteolysis; regulation of apoptosis
Research Articles on CASP12
Similar Products
Product Notes
The CASP12 casp12 (Catalog #AAA2027728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Caspase 12 (CASP12) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CASP12 casp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MAA RRTHERD PIYKIKGLAK DMLDGVFDDL VEKNVLNGDE LLKIGESASF ILNKAENLVE NFLEKTDMAG KIFAGHIANS QEQLSLQFSN DEDDGPQKIC TPSSPSESKR KVEDDEMEVN AGLAHESHLM LTAPHGLQSS EVQDTLKLCP RDQFCKIKTE RAKEIYPVME KEGRTRLALI ICNKKFDYLF DRDNADTDIL NMQELLENLG YSVVLKENLT AQEMETELMQ FAGRPEHQSS DSTFLVFMSH. It is sometimes possible for the material contained within the vial of "Caspase 12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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