Rabbit anti-Human CASP9 / Caspase 9 Polyclonal Antibody | anti-CASP9 antibody

Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9

Cat. Num. AAA243234

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CASP9 / Caspase 9; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9; Anti-CASP9 / Caspase 9 Antibody (aa266-315) IHC-plus; CASP9; APAF3; CASPASE-9c; ICE-LAP6; MCH6; ICE-like apoptotic protease 6; APAF-3; Apoptotic protease Mch-6; CASP-9; Caspase 9; Caspase-9; PPP1R56; Human CASP9; anti-CASP9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase 9 (Cleaved-Asp315) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp315.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 416

• Applicable Applications for anti-CASP9 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Caspase 9 (aa266-315).
• Immunogen: CASP9 / Caspase 9 antibody was raised against synthetic peptide from human Caspase 9 (aa266-315).
• Antigen Modification: aa266-315
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from 293 cells, treated with Etoposide 25 uM 60', using Caspase 9 (Cleaved-Asp315) Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-CASP9 antibody

Caspase 9 is a cysteine C14 endopeptidase that mediates oncogene and drug-induced apoptosis. It is activated upon binding to Apaf-1/cytochrome c and inhibited upon phosphorylation by the serine/ threonine kinase Akt. Activated caspase-9 cleaves downstream caspases such as Caspase-3, -6 and -7 initiating the caspase cascade. Like other caspases, Caspase-9 plays a critical role in mediating apoptosis during normal brain development.

Product Categories/Family for anti-CASP9 antibody

Family: Protease; Subfamily: Cysteine C14

NCBI and Uniprot Product Information

• NCBI GI #: 14790124
• NCBI GeneID: 842
• NCBI Accession #: NP_001220.2
• NCBI GenBank Nucleotide #: NM_001229.4
• UniProt Accession #: P55211; O95348; Q53Y70; Q5JRU9; Q5UGI1; Q92852; Q9BQ62; Q9UEQ3; Q9UIJ8; B4E1A3
• Molecular Weight: 36,564 Da
• NCBI Official Full Name: caspase-9 isoform alpha
• NCBI Official Synonym Full Names: caspase 9, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: EC 3.4.22.62; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: mitochondrion; nucleus; cytosol; apoptosome

Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; protein kinase binding; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging