Western Blot (WB)
(CASP5 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CASP5 antibody detected the CASP5 protein (arrow).)
Rabbit anti-Human CASP5 Polyclonal Antibody | anti-CASP5 antibody
CASP5 Antibody (Center)
Western Blot (WB)
(CASP5 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CASP5 antibody detected the CASP5 protein (arrow).)
Western Blot (WB)
(CASP5 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CASP5 antibody detected the CASP5 protein (arrow).)
Immunohistochemistry (IHC)
(CASP5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CASP5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CASP5 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CASP5 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
protease (caspase) family. Sequential activation of caspases plays
a central role in the execution-phase of cell apoptosis. Caspases
exist as inactive proenzymes which undergo proteolytic processing
at conserved aspartic residues to produce two subunits, large and
small, that dimerize to form the active enzyme. Overexpression of
the active form of this enzyme induces apoptosis in fibroblasts.
Max, a central component of the Myc/Max/Mad transcription
regulation network important for cell growth, differentiation, and
apoptosis, is cleaved by this protein; this process requires
Fas-mediated dephosphorylation of Max. The expression of this gene
is regulated by interferon-gamma and lipopolysaccharide.
Alternatively spliced transcript variants have been identified for
this gene.
Notaridou, M., et al. Int. J. Cancer (2010) In press :
Kim, M.S., et al. APMIS 118(4):308-312(2010)
Liang, X.S., et al. Br. J. Haematol. 146(4):418-423(2009)
Eckhart, L., et al. Biochem. Biophys. Res. Commun. 348(2):682-688(2006)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
Uniprot Description
CASP5: Mediator of programmed cell death (apoptosis). Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits. Up-regulated by bacterial lipopolysaccharides (LPS). Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.22.58
Chromosomal Location of Human Ortholog: 11q22.2-q22.3
Cellular Component: neuron projection; cell soma; cytoplasm
Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity
Biological Process: regulation of apoptosis; substantia nigra development; apoptosis; regulation of inflammatory response; germ cell programmed cell death; positive regulation of interleukin-1 beta secretion; proteolysis
Research Articles on CASP5
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Product Notes
The CASP5 casp5 (Catalog #AAA9213615) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 162-191. The CASP5 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CASP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the CASP5 casp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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