Rabbit anti-Human CASP2 Polyclonal Antibody | anti-CASP2 antibody

CASP2, ID (Caspase-2, CASP-2, ICH-1 Protease, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2, NEDD-2, Caspase-2 Subunit p18, Caspase-2 Subunit p13, Caspase-2 Subunit p12)

Cat. Num. AAA629020

• Gene Names: CASP2; ICH1; NEDD2; CASP-2; NEDD-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CASP2; Polyclonal Antibody; ID (Caspase-2; CASP-2; ICH-1 Protease; Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2; NEDD-2; Caspase-2 Subunit p18; Caspase-2 Subunit p13; Caspase-2 Subunit p12); Anti -CASP2; anti-CASP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CASP2.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CASP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the Center region of human CASP2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CASP2 antibody (Center) in HL60, K562, Ramos and NIH-3T3 cell line lysates(35ug/lane). CASP2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung with CASP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CASP2 Antibody (Center) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CASP2 antibody

CASP2 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. The protein exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli.

Product Categories/Family for anti-CASP2 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 39995059
• NCBI GeneID: 835
• NCBI Accession #: NP_116764.2
• NCBI GenBank Nucleotide #: NM_032982.3
• UniProt Accession #: P42575; P42576; Q59F21; Q7KZL6; Q86UJ3; Q9BUP7; Q9BZK9; A8K5F9; D3DXD6; Q9BZL0
• Molecular Weight: 50,685 Da
• NCBI Official Full Name: caspase-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: caspase 2, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP2
• NCBI Official Synonym Symbols: ICH1; NEDD2; CASP-2; NEDD-2
• NCBI Protein Information: caspase-2; protease ICH-1; OTTHUMP00000025259; OTTHUMP00000202227; neural precursor cell expressed developmentally down-regulated protein 2
• UniProt Protein Name: Caspase-2
• Protein Family: Caspase
• UniProt Gene Name: CASP2
• UniProt Synonym Gene Names: ICH1; NEDD2
• UniProt Entry Name: CASP2_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq]

Uniprot Description

CASP2: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD. Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.55; Protease

Chromosomal Location of Human Ortholog: 7q34-q35

Cellular Component: mitochondrion; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; cysteine-type endopeptidase activity

Biological Process: luteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; germ cell programmed cell death; proteolysis; regulation of caspase activity; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; protein processing; brain development; aging