Rabbit Casein Kinase IIbeta Polyclonal Antibody | anti-S205 antibody

Casein Kinase IIbeta (S205) Polyclonal Antibody

Cat. Num. AAA3001243

• Gene Names: CSNK2B; G5A; CK2B; CK2N; Ckb1; Ckb2; CSK2B; POBINDS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Casein Kinase IIbeta; Polyclonal Antibody; Casein Kinase IIbeta (S205) Polyclonal Antibody; Casein kinase II subunit beta; CK II beta; Phosvitin; Protein G5a; CSNK2B; CK2N; G5A; CKIIbeta; CK2B; anti-S205 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CK2-beta (S205) polyclonal antibody detects endogenous levels of CK2-beta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 215

• Applicable Applications for anti-S205 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 175-220 of Human Casein Kinase IIbeta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Casein Kinase IIbeta (S205) pAb at 1:500 dilutionLane1:HEK293 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:3T3-L1 whole cell lysate (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Casein Kinase IIbeta (S205) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of Casein Kinase IIbeta (S205) pAb at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (40ug))

Related Product Information for anti-S205 antibody

Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, gamma, epsilon and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The alpha catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

NCBI and Uniprot Product Information

• NCBI GI #: 23503295
• NCBI GeneID: 1460
• NCBI Accession #: NP_001311.3
• NCBI GenBank Nucleotide #: NP_001311.3
• UniProt Accession #: P67870
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: casein kinase II subunit beta isoform 1
• NCBI Official Synonym Full Names: casein kinase 2 beta
• NCBI Official Symbol: CSNK2B
• NCBI Official Synonym Symbols: G5A; CK2B; CK2N; Ckb1; Ckb2; CSK2B; POBINDS
• NCBI Protein Information: casein kinase II subunit beta
• UniProt Protein Name: Casein kinase II subunit beta
• UniProt Gene Name: CSNK2B
• UniProt Synonym Gene Names: CK2N; G5A; CK II beta
• UniProt Entry Name: CSK2B_HUMAN

NCBI Description

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

CK2B: a regulatory subunit of the casein kinase 2 holoenzyme. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. Plays a complex role in regulating the basal catalytic activity of the alpha subunit. Participates in Wnt signaling

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; plasma membrane; protein kinase CK2 complex; PcG protein complex; nucleus; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein domain specific binding; protein binding; metal ion binding; protein kinase regulator activity; transcription factor binding; receptor binding

Biological Process: negative regulation of cell proliferation; axon guidance; positive regulation of activin receptor signaling pathway; Wnt receptor signaling pathway; cellular protein complex assembly; regulation of DNA binding; adiponectin-mediated signaling pathway; mitotic cell cycle; regulation of protein kinase activity; negative regulation of blood vessel endothelial cell migration; signal transduction; protein amino acid phosphorylation