Western Blot (WB)
(Western Blot (WB) analysis of Casein Kinase I alpha (Q317) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (10ug) Lane2:A549 whole cell lysate (10ug) Lane3:The Lung tissue lysate of Rat (40ug) Lane4:The kidney tissue lysate of Mouse (40ug))
Rabbit Casein Kinase Ialpha Polyclonal Antibody | anti-Q317 antibody
Casein Kinase Ialpha (Q317) Polyclonal Antibody
Gene Names
CSNK1A1; CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Casein Kinase Ialpha; Polyclonal Antibody; Casein Kinase Ialpha (Q317) Polyclonal Antibody; CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1; CKIalpha; CK-Ialpha; CK Ialpha; anti-Q317 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Casein Kinase Ialpha (Q317) polyclonal antibody detects endogenous levels of Casein Kinase Ialpha protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
365
Applicable Applications for anti-Q317 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 300-350 of Human Casein Kinase Ialpha.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Casein Kinase I alpha (Q317) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (10ug) Lane2:A549 whole cell lysate (10ug) Lane3:The Lung tissue lysate of Rat (40ug) Lane4:The kidney tissue lysate of Mouse (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Casein Kinase I alpha (Q317) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (10ug) Lane2:A549 whole cell lysate (10ug) Lane3:The Lung tissue lysate of Rat (40ug) Lane4:The kidney tissue lysate of Mouse (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Casein Kinase Ialpha (Q317) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Casein Kinase Ialpha (Q317) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-Q317 antibody
Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, beta, gamma, e and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 42kDa
NCBI Official Full Name
casein kinase I isoform alpha isoform 1
NCBI Official Synonym Full Names
casein kinase 1 alpha 1
NCBI Official Symbol
CSNK1A1
NCBI Official Synonym Symbols
CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
NCBI Protein Information
casein kinase I isoform alpha
UniProt Protein Name
Casein kinase I isoform alpha
UniProt Gene Name
CSNK1A1
UniProt Synonym Gene Names
CKI-alpha
UniProt Entry Name
KC1A_HUMAN
Uniprot Description
CK1A: an ubiquitous protein kinase of the CK1 family. Participates in Wnt signaling. Phosphorylates beta catenin, marking it for beta-TRCp-mediated ubiquitinylation and proteasomal degradation.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CK1; EC 2.7.11.1; CK1 group; CK1 family
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: centrosome; membrane; cytoplasm; nuclear speck; mRNA cleavage and polyadenylation specificity factor complex; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: mitosis; regulation of cell shape; peptidyl-serine phosphorylation; cell surface receptor linked signal transduction; Wnt receptor signaling pathway; cell division; signal transduction; protein amino acid phosphorylation; phagocytosis
Research Articles on Q317
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Product Notes
The Q317 csnk1a1 (Catalog #AAA3001762) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Casein Kinase Ialpha (Q317) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Casein Kinase Ialpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the Q317 csnk1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Casein Kinase Ialpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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