Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human testis tissue, K562 cells, Primary antibody: 821598(CARF Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
Rabbit anti-Human CARF Polyclonal Antibody | anti-CARF antibody
CARF Rabbit pAb
Gene Names
CARF; ALS2CR8; NYD-SP24
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
CARF; Polyclonal Antibody; CARF Rabbit pAb; ALS2CR8; NYD-SP24; anti-CARF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-CARF antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:200-1:1000
Immunogen
Fusion protein of human CARF
Conjugation
Unconjugated
Molecular Function
CARF (calcium-response factor), also known as ALS2CR8 (amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein) or NYD-SP24, is a 725 amino acid nuclear protein. Expressed in a wide variety of tissues with highest expression in the hippocampus, CARF is thought to be a transcription factor that associates with the p53 tumor suppression pathway. CARF cooperates, co-localizes and is co-regulated with ARF, an ADP-ribosylation factor, and, through this interaction, helps to mediate ARF-p53-induced apoptotic signaling. This apoptotic pathway is implicated in cell cycle control, proper cellular development, response to DNA damage and the aging process, suggesting that CARF participates in various events throughout the cell.
Modification
Unmodified
Cell Position
Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human testis tissue, K562 cells, Primary antibody: 821598(CARF Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human testis tissue, K562 cells, Primary antibody: 821598(CARF Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
Product Categories/Family for anti-CARF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
72,385 Da
NCBI Official Full Name
Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8, mRNA
NCBI Official Synonym Full Names
calcium responsive transcription factor
NCBI Official Symbol
CARF
NCBI Official Synonym Symbols
ALS2CR8; NYD-SP24
NCBI Protein Information
calcium-responsive transcription factor
UniProt Protein Name
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein
Protein Family
UniProt Gene Name
ALS2CR8
UniProt Synonym Gene Names
CARF; CaRF
UniProt Entry Name
AL2S8_HUMAN
Uniprot Description
CARF: May be a transcription factor. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 2q33.2
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: DNA binding; transcription factor activity
Biological Process: transcription, DNA-dependent
Research Articles on CARF
Similar Products
Product Notes
The CARF als2cr8 (Catalog #AAA4757884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARF Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CARF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the CARF als2cr8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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