Western Blot (WB)
((0.1ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CARD15/NOD2 Polyclonal Antibody | anti-CARD15 antibody
Goat anti-CARD15/NOD2 (Internal) Antibody
Western Blot: Approx 100kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115kDa according to NP_071445.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa.
Western Blot (WB)
((0.1ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Uniprot Description
NOD2: Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK- gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash. Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1). IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Defects in NOD2 are the cause of sarcoidosis early-onset (EOS). EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early- onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement. 2 isoforms of the human protein are produced by alternative initiation.
Chromosomal Location of Human Ortholog: 16q21
Cellular Component: basolateral plasma membrane; cell surface; cytoplasm; cytoskeleton; cytosol; plasma membrane; protein complex; signalosome; vesicle
Molecular Function: actin binding; ATP binding; CARD domain binding; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; muramyl dipeptide binding; peptidoglycan binding; protein binding; protein kinase binding
Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; cytokine production during immune response; cytokine secretion during immune response; defense response; defense response to bacterium; detection of bacterium; detection of biotic stimulus; detection of muramyl dipeptide; innate immune response; JNK cascade; maintenance of gastrointestinal epithelium; positive regulation of B cell activation; positive regulation of dendritic cell antigen processing and presentation; positive regulation of epithelial cell proliferation; positive regulation of gamma-delta T cell activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-10 production; positive regulation of interleukin-17 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of Notch signaling pathway; positive regulation of oxidoreductase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of stress-activated MAPK cascade; positive regulation of T-helper 2 type immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; protein oligomerization; regulation of inflammatory response; response to muramyl dipeptide
Disease: Blau Syndrome; Inflammatory Bowel Disease 1; Psoriatic Arthritis, Susceptibility To; Sarcoidosis, Early-onset
Research Articles on CARD15
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Product Notes
The CARD15 nod2 (Catalog #AAA420357) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CARD15/NOD2 (Internal) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CARD15/NOD2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 100kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115kDa according to NP_071445.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. Researchers should empirically determine the suitability of the CARD15 nod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KFRFTDRERH CSPTD. It is sometimes possible for the material contained within the vial of "CARD15/NOD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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