Western Blot (WB)
(Western blot analysis of CARD10 using HeLa whole cell lysates)
Rabbit anti-Human, Mouse CARD10 Polyclonal Antibody | anti-CARD10 antibody
CARD10 Antibody
Gene Names
CARD10; BIMP1; CARMA3
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CARD10; Polyclonal Antibody; CARD10 Antibody; Bcl10 binding protein and activator of NFKB; BIMP1; CAR10_HUMAN; CARD 10; CARD containing MAGUK 3 protein; CARD-containing MAGUK 3; CARD-containing MAGUK protein 3; Carma 3; CARMA3; Caspase Recruitment Domain family member 10; Caspase recruitment domain-containing protein 10; anti-CARD10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CARD10 antibody detects endogenous levels of total CARD10
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1032
Applicable Applications for anti-CARD10 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human CARD10
Subcellular Location
Cytoplasm.
Tissue Specificity
Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CARD10 using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis of CARD10 using HeLa whole cell lysates)
Immunofluorescene (IF)
(MBS9608959 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9608959 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CARD10 antibody
Function: Activates NF-kappa-B via BCL10 and IKK.
Subunit Structure: CARD10 and BCL10 bind to each other by CARD-CARD interaction. They both participate in a complex with MALT1, where MALT1 binds to BCL10 (By similarity).
Subunit Structure: CARD10 and BCL10 bind to each other by CARD-CARD interaction. They both participate in a complex with MALT1, where MALT1 binds to BCL10 (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 115 kDa
Predicted: 116 kDa
Predicted: 116 kDa
NCBI Official Full Name
caspase recruitment domain-containing protein 10
NCBI Official Synonym Full Names
caspase recruitment domain family member 10
NCBI Official Symbol
CARD10
NCBI Official Synonym Symbols
BIMP1; CARMA3
NCBI Protein Information
caspase recruitment domain-containing protein 10
UniProt Protein Name
Caspase recruitment domain-containing protein 10
Protein Family
UniProt Gene Name
CARD10
UniProt Synonym Gene Names
CARMA3; Carma 3
NCBI Description
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]
Uniprot Description
Activates NF-kappa-B via BCL10 and IKK.CautionSupposed to contain a SH3, a PDZ and a guanylate kinase-like domain. But none of these 3 domains are detected by PROSITE, Pfam or SMART.
Research Articles on CARD10
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Product Notes
The CARD10 card10 (Catalog #AAA9608959) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARD10 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CARD10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CARD10 card10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARD10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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