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Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1.5ug/ml Rabbit Anti-Mouse CA5B Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Mouse Carbonic Anhydrase VB, Mitochondrial (CA5B) Polyclonal Antibody | anti-CA5B antibody
Polyclonal Antibody to Carbonic Anhydrase VB, Mitochondrial (CA5B)
Gene Names
Car5b; CAVB; Ca5b; CarVb; 7330410H16Rik; D730005F19Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Carbonic Anhydrase VB; Mitochondrial (CA5B); Polyclonal Antibody; Polyclonal Antibody to Carbonic Anhydrase VB; anti-CA5B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CA5B. It has been selected for its ability to recognize CA5B in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-RQSPINIR WRDSVYDPGL KPLTISYDPA TCLHIWNNGY SFLVEFEDST DKSVVEGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCY PAELHLVHWN AVKFESFEDA ALEENGLAVI GVFLKLGKHH KELQKLVDTL PSIKHKDTLV EFGSFDPSCL MPTCP
MGHHHHHHSGSEF-RQSPINIR WRDSVYDPGL KPLTISYDPA TCLHIWNNGY SFLVEFEDST DKSVVEGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCY PAELHLVHWN AVKFESFEDA ALEENGLAVI GVFLKLGKHH KELQKLVDTL PSIKHKDTLV EFGSFDPSCL MPTCP
Sequence Length
317
Applicable Applications for anti-CA5B antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CA5B (Arg63~Pro225) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2069808
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1.5ug/ml Rabbit Anti-Mouse CA5B Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1.5ug/ml Rabbit Anti-Mouse CA5B Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,623 Da
NCBI Official Full Name
carbonic anhydrase 5B, mitochondrial
NCBI Official Synonym Full Names
carbonic anhydrase 5b, mitochondrial
NCBI Official Symbol
Car5b
NCBI Official Synonym Symbols
CAVB; Ca5b; CarVb; 7330410H16Rik; D730005F19Rik
NCBI Protein Information
carbonic anhydrase 5B, mitochondrial
UniProt Protein Name
Carbonic anhydrase 5B, mitochondrial
Protein Family
UniProt Gene Name
Ca5b
UniProt Synonym Gene Names
Car5b; CA-VB
Uniprot Description
CA5B: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.
Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Mitochondrial
Chromosomal Location of Human Ortholog: X|X F5
Cellular Component: mitochondrion
Molecular Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding
Biological Process: one-carbon metabolic process
Research Articles on CA5B
Similar Products
Product Notes
The CA5B ca5b (Catalog #AAA2004341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Carbonic Anhydrase VB, Mitochondrial (CA5B) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase VB, Mitochondrial (CA5B) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CA5B ca5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-RQSPIN IR WRDSVYDPGL KPLTISYDPA TCLHIWNNGY SFLVEFEDST DKSVVEGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCY PAELHLVHWN AVKFESFEDA ALEENGLAVI GVFLKLGKHH KELQKLVDTL PSIKHKDTLV EFGSFDPSCL MPTCP. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase VB, Mitochondrial (CA5B), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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