Testing Data
(Detection of carbonic anhydrase IX in transfected HEK cell lysate using MBS616120. Rabbit IgG was used as a negative control.)
Rabbit Carbonic Anhydrase IX Polyclonal Antibody | anti-CA9 antibody
Carbonic Anhydrase IX (CA9)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Other applications not tested.
Western Blot: 1:2000
Flow Cytometry: 1:1000
Immunohistochemistry (Frozen, paraffin): 1:1000.
Optimal dilutions to be determined by the researcher
Testing Data
(Detection of carbonic anhydrase IX in transfected HEK cell lysate using MBS616120. Rabbit IgG was used as a negative control.)
Testing Data
(Detection of carbonic anhydrase IX in transfected HEK cell lysate using MBS616120. Rabbit IgG was used as a negative control.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer usingMBS616120 at 1:1000 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer usingMBS616120 at 1:1000 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemistry of frozen human colon carcinoma, xenografted in mice using MBS616120)
Immunohistochemistry (IHC)
(Immunohistochemistry of frozen human colon carcinoma, xenografted in mice using MBS616120)
Immunofluorescence (IF)
(Immunofluorescence of HEK 293 cells using MBS616120. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.)
Immunofluorescence (IF)
(Immunofluorescence of HEK 293 cells using MBS616120. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.)
NCBI and Uniprot Product Information
NCBI Description
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]
Uniprot Description
CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.
Protein type: Membrane protein, integral; Nucleolus; Energy Metabolism - nitrogen; Lyase; EC 4.2.1.1
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: basolateral plasma membrane; microvillus membrane; nucleolus; integral to membrane; plasma membrane
Molecular Function: carbonate dehydratase activity; zinc ion binding
Biological Process: response to drug; secretion; bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to testosterone stimulus
Research Articles on CA9
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Product Notes
The CA9 ca9 (Catalog #AAA616120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Carbonic Anhydrase IX (CA9) reacts with Canine, mouse and rat and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase IX can be used in a range of immunoassay formats including, but not limited to, Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Flow Cytometry and Immunohistochemistry.Other applications not tested. Immunofluorescence (IC): 1:1000 Western Blot: 1:2000 Flow Cytometry: 1:1000 Immunohistochemistry (Frozen, paraffin): 1:1000. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CA9 ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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