Rabbit anti-Human CAPZA1 Polyclonal Antibody | anti-CAPZA1 antibody

CAPZA1, NT (F-actin-capping Protein Subunit alpha-1, CapZ alpha-1) (AP)

Cat. Num. AAA6461213

• Gene Names: CAPZA1; CAPZ; CAZ1; CAPPA1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CAPZA1; Polyclonal Antibody; NT (F-actin-capping Protein Subunit alpha-1; CapZ alpha-1) (AP); anti-CAPZA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CAPZA1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CAPZA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CAPZA1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CAPZA1 antibody (N-term) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Flow Cytometry (FC/FACS)

(CAPZA1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CAPZA1 antibody

Antibodies; Actin Related Proteins

References

1. Maun, N.A., Speicher, D.W. Biochemistry 35(11), 3518-3524 (1996). 2. Barron-Casella, E.A., Torres, M.A. J. Biol. Chem. 270(37), 21472-21479 (1995). 3. Canton, D.A., Olsten, M.E. J. Biol. Chem. 281(47), 36347-36359 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5453597
• NCBI GeneID: 829
• NCBI Accession #: NP_006126
• NCBI GenBank Nucleotide #: NM_006135
• UniProt Accession #: P52907; Q53FQ6; Q6FHD5
• Molecular Weight: 32,923 Da
• NCBI Official Full Name: F-actin-capping protein subunit alpha-1
• NCBI Official Synonym Full Names: capping protein (actin filament) muscle Z-line, alpha 1
• NCBI Official Symbol: CAPZA1
• NCBI Official Synonym Symbols: CAPZ; CAZ1; CAPPA1
• NCBI Protein Information: F-actin-capping protein subunit alpha-1; Cap Z; capZ alpha-1
• UniProt Protein Name: F-actin-capping protein subunit alpha-1
• Protein Family: F-actin-capping protein
• UniProt Gene Name: CAPZA1
• UniProt Entry Name: CAZA1_HUMAN

Uniprot Description

CAPZA1: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Belongs to the F-actin-capping protein alpha subunit family.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: F-actin capping protein complex; cytoskeleton; extracellular region; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: innate immune response; protein complex assembly; cell motility; blood coagulation; barbed-end actin filament capping