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Western Blot (WB) (Western Blot Test: E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Chicken anti-Human, Mouse CAPG Polyclonal Antibody | anti-CAPG antibody

CAPG (Capping Protein Gelsolin-Like, Actin Filament)

Gene Names
CAPG; MCP; AFCP
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
CAPG; Polyclonal Antibody; CAPG (Capping Protein Gelsolin-Like; Actin Filament); Anti -CAPG (Capping Protein Gelsolin-Like; anti-CAPG antibody
Ordering
For Research Use Only!
Host
Chicken
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes human Capping Protein (Actin filament) Gelsolin-like. Species Crossreactivity: Human and mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.
Applicable Applications for anti-CAPG antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Flow Cytometry: 1:200
Immunohistochemistry: 1:200
Immunogen
Synthetic peptide corresponding to aa1-348
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Test: E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Western Blot (WB) (Western Blot Test: E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)
Related Product Information for anti-CAPG antibody
This protein, first purified from rabbit alveolar macrophages, was named macrophage capping protein. The protein was subsequently renamed Cap G (gene symbol CAPG). 'Cap' was chosen because unlike other members of the gelsolin/villin family, this protein caps the barbed ends of actin filaments but does not sever them. 'G' was included in the name because the derived amino acid sequence of Cap G most closely resembles gelsolin, possessing 49% identity with its amino terminal half.
Product Categories/Family for anti-CAPG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
822
UniProt Accession #
Molecular Weight
38,499 Da
NCBI Official Full Name
capping protein (actin filament), gelsolin-like, isoform CRA_a
NCBI Official Synonym Full Names
capping protein (actin filament), gelsolin-like
NCBI Official Symbol
CAPG
NCBI Official Synonym Symbols
MCP; AFCP
NCBI Protein Information
macrophage-capping protein; OTTHUMP00000203056; OTTHUMP00000203057; OTTHUMP00000203058; OTTHUMP00000203059; OTTHUMP00000203060; OTTHUMP00000203063; macrophage capping protein; gelsolin-like capping protein; actin regulatory protein CAP-G; actin-regulatory protein CAP-G
UniProt Protein Name
Macrophage-capping protein
Protein Family
UniProt Gene Name
CAPG
UniProt Synonym Gene Names
AFCP; MCP
UniProt Entry Name
CAPG_HUMAN

NCBI Description

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq]

Uniprot Description

CAPG: Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. Belongs to the villin/gelsolin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: F-actin capping protein complex; melanosome; nucleus

Molecular Function: actin binding

Biological Process: protein complex assembly; cell projection biogenesis; barbed-end actin filament capping

Research Articles on CAPG

Similar Products

Product Notes

The CAPG capg (Catalog #AAA614342) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The CAPG (Capping Protein Gelsolin-Like, Actin Filament) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CAPG can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Dilution: Western Blot: 1:1000 Flow Cytometry: 1:200 Immunohistochemistry: 1:200. Researchers should empirically determine the suitability of the CAPG capg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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