Rabbit anti-Human, Mouse CAMKK1 Polyclonal Antibody | anti-CAMKK1 antibody

CAMKK1, ID (Calcium/Calmodulin-dependent Protein Kinase Kinase 1, CaM-kinase Kinase 1, CaM-KK 1, CaMKK 1, CaMKK 1, Calcium/Calmodulin-dependent Protein Kinase Kinase alpha, CaM-kinase Kinase alpha, CaM-KK alpha, CaMKK alpha, CaM-kinase IV Kinase, CAMKKA)

Cat. Num. AAA6466894

• Gene Names: CAMKK1; CAMKKA
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CAMKK1; Polyclonal Antibody; ID (Calcium/Calmodulin-dependent Protein Kinase Kinase 1; CaM-kinase Kinase 1; CaM-KK 1; CaMKK 1; Calcium/Calmodulin-dependent Protein Kinase Kinase alpha; CaM-kinase Kinase alpha; CaM-KK alpha; CaMKK alpha; CaM-kinase IV Kinase; CAMKKA); anti-CAMKK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CAMKK1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 505

• Applicable Applications for anti-CAMKK1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CAMKK1 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CAMKK1 Antibody (Center) in mouse thymus tissue lysates (35ug/lane). CAMKK1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CAMKK1 (arrow) using rabbit polyclonal CAMKK1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMKK1 gene (Lane 2).)

Product Categories/Family for anti-CAMKK1 antibody

Antibodies; Calmodulin

References

1. Guest, C.B., PLoS ONE 3(2), E1606 (2008). 2. Rudd, M.F., Genome Res. 16(6), 693-701 (2006). 3. Ishikawa, Y., FEBS Lett. 550(1-3), 57-63 (2003). 4. Matsushita, M., J. Biol. Chem. 274(15), 10086-10093 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 14150045
• NCBI GeneID: 84254
• NCBI Accession #: NP_115670
• NCBI GenBank Nucleotide #: NP_115670.1
• UniProt Accession #: Q8N5S9
• Molecular Weight: 55.735
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase kinase 1 isoform a
• NCBI Official Synonym Full Names: calcium/calmodulin dependent protein kinase kinase 1
• NCBI Official Symbol: CAMKK1
• NCBI Official Synonym Symbols: CAMKKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase kinase 1
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase kinase 1
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMKK1
• UniProt Synonym Gene Names: CAMKKA; CaM-KK 1; CaM-kinase kinase 1; CaMKK 1; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha
• UniProt Entry Name: KKCC1_HUMAN

NCBI Description

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMKK1: a calcium/calmodulin-dependent S/T protein kinase of the CAMKK family. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. 2 isoforms of the human protein are produced by alternative splicing. The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition. Binding of calmodulin may releave intrasteric autoinhibition. May be regulated through phosphorylation by CAMK1 and CAMK4. Appears to be autophosphorylated in a Ca2+/calmodulin-dependent manner. Phosphorylation of Ser-458 is blocked upon binding to Ca2+/calmodulin.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; EC 2.7.11.17; Other group; CAMKK family; Meta subfamily

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: synaptic transmission; protein amino acid phosphorylation