Rabbit anti-Human CAMK2D Polyclonal Antibody | anti-CAMK2D antibody

CAMK2D, CT (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit delta, CaM-kinase II Subunit delta, CaMK-II Subunit delta, CAMKD) (Azide free) (HRP)

Cat. Num. AAA6466815

• Gene Names: CAMK2D; CAMKD
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CAMK2D; Polyclonal Antibody; CT (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit delta; CaM-kinase II Subunit delta; CaMK-II Subunit delta; CAMKD) (Azide free) (HRP); anti-CAMK2D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CAMK2 delta. Species Crossreactivity: primate.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CAMK2D antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CAMK2 delta (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-CAMK2delta C-term Pab is used in Western blot to detect CAMK2 delta in serum-starved HeLa cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2 delta Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CAMK2D antibody

Antibodies; Calmodulin

References

1. Rochlitz, H., et al., Diabetologia 43(4):465-473 (2000). 2. Hoch, B., et al., Circ. Res. 84(6):713-721 (1999). 3. Tombes, R.M., et al., Biochim. Biophys. Acta 1355(3):281-292 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 26667180
• NCBI GeneID: 817
• NCBI Accession #: NP_001212
• NCBI GenBank Nucleotide #: NM_001221
• UniProt Accession #: Q13557; Q52PK4; Q59G21; Q8N553; Q9UGH6; Q9UQE9; A8MVS8
• Molecular Weight: 54,114 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit delta isoform 3
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II delta
• NCBI Official Symbol: CAMK2D
• NCBI Official Synonym Symbols: CAMKD
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II delta subunit; CaM-kinase II delta chain; CaMK-II delta subunit; caM kinase II subunit delta; caMK-II subunit delta; calcium/calmodulin-dependent protein kinase (CaM kinase)
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit delta
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2D
• UniProt Synonym Gene Names: CAMKD; CaM kinase II subunit delta; CaMK-II subunit delta
• UniProt Entry Name: KCC2D_HUMAN

Uniprot Description

CAMK2D: a protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group that interacts with the sarcoplasmic reticulum membrane in cardiac and skeletal muscle. Regulates Ca2+ homeostatis and excitation-contraction coupling (ECC) in heart. Calmodulin binding induces conformational changes that relieve autoinhibition, permitting autophosphorylation at T287, rendering the kinase constitutively active and independent of Ca2+-binding. Targets ion channels, transporters and accessory proteins involved in Ca2+ influx into the myocyte, Ca2+ release from the sarcoplasmic reticulum (SR), SR Ca2+ uptake and Na+ and K+ channel transport. Also targets transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca2+ release via direct phosphorylation of RYR2 Ca2+ channel on S2808. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca2+ influx to myocytes by binding and phosphorylating the L-type Ca2+ channel subunit beta-2 CACNB2. In addition to Ca2+ channels, can target and regulate the cardiac sarcolemmal Na+ channel SCN5A and the K+ channel Kv4.3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLB), an endogenous inhibitor of SERCA2A, contributing to the enhancement of SR Ca2+ uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca2+ transport through phosphorylation of PLB and TRDN, a ryanodine receptor-coupling factor. CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with RRAD and CACNB2. Ten isoforms of the human protein are produced by alternative splicing. Isoform Delta3, isoform Delta2, isoform Delta8 and isoform Delta9 are expressed in cardiac muscle. Isoform Delta11 is expressed in skeletal muscle. Activity is induced in skeletal muscle during exercise. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Protein type: EC 2.7.11.17; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; cytosol; sarcolemma

Molecular Function: calmodulin binding; sodium channel inhibitor activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; titin binding; nitric-oxide synthase binding; ATP binding

Biological Process: positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; peptidyl-threonine phosphorylation; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; cellular potassium ion homeostasis; regulation of heart contraction; protein oligomerization; regulation of transcription from RNA polymerase II promoter; positive regulation of Rac protein signal transduction; synaptic transmission; endoplasmic reticulum calcium ion homeostasis; peptidyl-serine phosphorylation; regulation of G2/M transition of mitotic cell cycle; response to hypoxia; regulation of cell growth; regulation of the force of heart contraction