Rabbit anti-Human CAMK2A Polyclonal Antibody | anti-CAMK2A antibody

CAMK2A, CT (E370) (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM-kinase II Subunit alpha, CaM Kinase II Subunit alpha, CAMKA, KIAA0968) (PE)

Cat. Num. AAA6466810

• Gene Names: CAMK2A; CAMKA
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CAMK2A; Polyclonal Antibody; CT (E370) (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha; CaM-kinase II Subunit alpha; CaM Kinase II Subunit alpha; CAMKA; KIAA0968) (PE); anti-CAMK2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CAMK2A.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CAMK2A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CAMK2A (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CAMK2A Antibody (C-term E370) in 293 cell line lysates (35ug/lane). CAMK2A(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CAMK2A (arrow) using rabbit polyclonal CAMK2A Antibody (C-term E370). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK2A gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2A (C-term E370), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CAMK2A antibody

Antibodies; Calmodulin

References

1. Lee, C.W., Mol. Pharmacol. 73(5), 1454-1464 (2008). 2. Yuan, K., Lab. Invest. 87(9), 938-950 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 25952114
• NCBI GeneID: 815
• NCBI Accession #: NP_057065
• NCBI GenBank Nucleotide #: NM_015981
• UniProt Accession #: Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352
• Molecular Weight: 55,320 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 1
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha subunit; CaM-kinase II alpha chain; CaMK-II alpha subunit; CaMKIINalpha; caM kinase II subunit alpha; caMK-II subunit alpha; calcium/calmodulin-dependent protein kinase
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha
• UniProt Entry Name: KCC2A_HUMAN

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: nucleoplasm; presynaptic membrane; mitochondrion; plasma membrane; cytosol; cell junction; nucleus

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; G1/S transition of mitotic cell cycle; activation of NF-kappaB transcription factor