Rabbit CAMK2A Polyclonal Antibody | anti-CAMK2A antibody

Phospho-CAMK2A (Thr305) Antibody

Cat. Num. AAA7133381

• Gene Names: CAMK2A; CAMKA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Synonyms: CAMK2A; Polyclonal Antibody; Phospho-CAMK2A (Thr305) Antibody; KCC2A; kinase CaMK2-alpha; CaMKII-alpha; anti-CAMK2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Form/Format: Liquid; Rabbit IgG In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-CAMK2A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: Peptide sequence around phosphorylation site of threonine 305 (I-L-T(p)-T-M) derived from Human CaMK2alpha/beta/delta.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from 3T3 cells (Lane 2), using CaMK2alpha/beta/delta (Phospho-Thr305) Antibody. The lane on the left is treated with antigen-specific peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Product Categories/Family for anti-CAMK2A antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 25952118
• NCBI GeneID: 815
• NCBI Accession #: NP_741960.1
• NCBI GenBank Nucleotide #: NM_171825.2
• UniProt Accession #: Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352
• Molecular Weight: 54,088 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; CaMKIINalpha; CaMK-II alpha subunit; caMK-II subunit alpha; CaM-kinase II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; calcium/calmodulin-dependent protein kinase
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha
• UniProt Entry Name: KCC2A_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: nucleoplasm; presynaptic membrane; mitochondrion; plasma membrane; cytosol; cell junction; nucleus

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; G1/S transition of mitotic cell cycle; activation of NF-kappaB transcription factor