Immunohistochemistry
Rabbit Calretinin Polyclonal Antibody | anti-Calretinin antibody
Rabbit anti Calretinin Polyclonal
Reactivity
Human, Mouse, Rat, Cow
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Serum IgG fraction was purified by Protein G affinity chromatography.
Synonyms
Calretinin; Polyclonal Antibody; Rabbit anti Calretinin Polyclonal; Rabbit anti Calretinin Polyclonal purified; cal2b; polyclonal; Glycogen synthase kinase; anti-Calretinin antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Cow
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum IgG fraction was purified by Protein G affinity chromatography.
Form/Format
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Sequence Length
271
Applicable Applications for anti-Calretinin antibody
Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
Application Notes
ELISA: Use at a concentration range of 1-2ug/ml.
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of Calretinin-expressing Human uterine SMC cells.
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of Calretinin-expressing Human uterine SMC cells.
Source Note
Polyclonal Calretinin antibody was produced from sera of mice immunized with synthetic protein containing Calretinin antigenic determinants.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Preparation and Storage
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Immunohistochemistry
Immunohistochemistry
Product Categories/Family for anti-Calretinin antibody
References
• decrease of the immunohistochemical expression of calcium binding protein (calretinin) in the retina of hypercholesterolemic rabbits treated with roziglitazone .Invest. Ophthalmol. Vis. Sci., Apr 2010; 51: 6148.
• cxcr4 is required for proper regional and laminar distribution of cortical somatostatin-, calretinin-, and neuropeptide y-expressing gabaergic interneurons .Cereb Cortex, Mar 2010; 10.1093/cercor/bhq027.
• two calretinin-positive gabaergic cell types in layer 2/3 of the mouse neocortex provide different forms of inhibition .Cereb Cortex, Jun 2009; 19: 1345 - 1359.
• sv40-induced expression of calretinin protects mesothelial cells from asbestos cytotoxicity and may be a key factor contributing to mesothelioma pathogenesis .Am. J. Pathol., Jun 2009; 174: 2324 - 2336.
• the importance of calretinin in the detection of early alterations of the sensory retina in hypercholesterolemia experimental model .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4941.
• cxcr4 is required for proper regional and laminar distribution of cortical somatostatin-, calretinin-, and neuropeptide y-expressing gabaergic interneurons .Cereb Cortex, Mar 2010; 10.1093/cercor/bhq027.
• two calretinin-positive gabaergic cell types in layer 2/3 of the mouse neocortex provide different forms of inhibition .Cereb Cortex, Jun 2009; 19: 1345 - 1359.
• sv40-induced expression of calretinin protects mesothelial cells from asbestos cytotoxicity and may be a key factor contributing to mesothelioma pathogenesis .Am. J. Pathol., Jun 2009; 174: 2324 - 2336.
• the importance of calretinin in the detection of early alterations of the sensory retina in hypercholesterolemia experimental model .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4941.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,540 Da
NCBI Official Full Name
calretinin isoform 1
UniProt Protein Name
Calretinin
Protein Family
UniProt Gene Name
CALB2
UniProt Synonym Gene Names
CAB29; CR
UniProt Entry Name
CALB2_HUMAN
NCBI Description
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Uniprot Description
CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 16q22.2
Cellular Component: cytoplasm; gap junction; nucleus
Molecular Function: calcium ion binding
Biological Process: cytosolic calcium ion homeostasis
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Product Notes
The Calretinin calb2 (Catalog #AAA551074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Calretinin Polyclonal reacts with Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Calretinin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA). ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of Calretinin-expressing Human uterine SMC cells. Researchers should empirically determine the suitability of the Calretinin calb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calretinin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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