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Western Blot (WB) (Western Blot: Sample: Lane1: Human Serum; Lane2: Porcine Brain Tissue; Lane3: Mouse Brain Tissue; Lane4: Rat Brain Tissue.)

Rabbit anti-Human Calretinin Polyclonal Antibody | anti-CR antibody

Polyclonal Antibody to Calretinin (CR)

Gene Names
CALB2; CR; CAL2; CAB29
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Calretinin; Polyclonal Antibody; Polyclonal Antibody to Calretinin (CR); anti-CR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-CR antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200

Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant Calretinin (Phe28~Met248 (Accession # P22676)) expressed in E.coli
Cross Reactivity
Human, Mouse, Rat, Pig
Conjugate
No Conjugate
Immunogen
Recombinant calretinin (Phe28~Met248) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2046032
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human Serum; Lane2: Porcine Brain Tissue; Lane3: Mouse Brain Tissue; Lane4: Rat Brain Tissue.)

Western Blot (WB) (Western Blot: Sample: Lane1: Human Serum; Lane2: Porcine Brain Tissue; Lane3: Mouse Brain Tissue; Lane4: Rat Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant calretinin, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant calretinin, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Brain Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Brain Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
794
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
calretinin isoform 1
NCBI Official Synonym Full Names
calbindin 2
NCBI Official Symbol
CALB2
NCBI Official Synonym Symbols
CR; CAL2; CAB29
NCBI Protein Information
calretinin
UniProt Protein Name
Calretinin
Protein Family
UniProt Gene Name
CALB2
UniProt Synonym Gene Names
CAB29; CR

NCBI Description

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: cytosol; gap junction; neuron projection; nucleus; synapse; terminal bouton

Molecular Function: calcium ion binding

Biological Process: regulation of cytosolic calcium ion concentration

Research Articles on CR

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Product Notes

The CR calb2 (Catalog #AAA2028731) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Calretinin (CR) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calretinin can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 ug/mL;1:500-2000 Immunohistochemistry: 5-20 ug/mL;1:50-200 Immunocytochemistry: 5-20 ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CR calb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calretinin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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