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Western Blot (WB) (Anti-CNN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:37 kDa)

Rabbit Calponin/CNN1 Polyclonal Antibody | anti-CNN1 antibody

Anti-Calponin/CNN1 Antibody, Rabbit Polyclonal

Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Calponin/CNN1; Polyclonal Antibody; Anti-Calponin/CNN1 Antibody; Rabbit Polyclonal; calponin 1/CNN1 Antibody; Rabbit PAb; Antigen Affinity Purified; calponin 1; basic; smooth muscle; Anti-HEL-S-14 Antibody; Anti-Sm-Calp Antibody; Anti-SMCC Antibody; anti-CNN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Calponin/CNN1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CNN1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:5000-1:10,000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human calponin 1/CNN1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human calponin 1/CNN1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CNN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:37 kDa)

Western Blot (WB) (Anti-CNN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:37 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human CNN1 in human breast with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human CNN1 in human breast with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CNN1 in human prostate with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human CNN1 in human prostate with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Product Categories/Family for anti-CNN1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,055 Da
NCBI Official Full Name
calponin-1
NCBI Official Synonym Full Names
calponin 1, basic, smooth muscle
NCBI Official Symbol
CNN1
NCBI Official Synonym Symbols
SMCC; Sm-Calp; HEL-S-14
NCBI Protein Information
calponin-1; basic calponin; calponin H1, smooth muscle; calponins, basic; epididymis secretory protein Li 14
UniProt Protein Name
Calponin-1
Protein Family
UniProt Gene Name
CNN1
UniProt Entry Name
CNN1_HUMAN

Uniprot Description

calponin 1: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.

Protein type: Actin-binding; Contractile; Myosin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: focal adhesion; cytoskeleton

Molecular Function: calmodulin binding; actin binding

Biological Process: regulation of smooth muscle contraction; actomyosin structure organization and biogenesis

Research Articles on CNN1

Similar Products

Product Notes

The CNN1 cnn1 (Catalog #AAA8101767) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Calponin/CNN1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Calponin/CNN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 IHC-P: 1:5000-1:10,000. Researchers should empirically determine the suitability of the CNN1 cnn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calponin/CNN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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