Rabbit anti-Human Calponin-3 Polyclonal Antibody | anti-CNN3 antibody

Calponin-3, ID (Calponin 3, Calponin, Acidic Isoform, CNN3) (MaxLight 490)

Cat. Num. AAA6466872

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Calponin-3; Polyclonal Antibody; ID (Calponin 3; Calponin; Acidic Isoform; CNN3) (MaxLight 490); anti-CNN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Calponin-3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-CNN3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human Calponin-3 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Calponin-3 antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Calponin-3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Calponin-3 Antibody (Center) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CNN3 antibody

Antibodies; Cancer Markers

References

1. Vinatzer, U., Clin. Cancer Res. 14(20), 6426-6431 (2008). 2. Haag, J., Exp. Cell Res. 313(16), 3386-3394 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4502923
• NCBI GeneID: 1266
• NCBI Accession #: NP_001830
• NCBI GenBank Nucleotide #: NM_001839
• UniProt Accession #: Q15417; Q6FHA7; B4DFK6; B4DP09; F8WA86
• Molecular Weight: 31,380 Da
• NCBI Official Full Name: calponin-3 isoform 1
• NCBI Official Synonym Full Names: calponin 3, acidic
• NCBI Official Symbol: CNN3
• NCBI Protein Information: calponin-3; calponin, acidic isoform; dJ639P13.2.2 (acidic calponin 3)
• UniProt Protein Name: Calponin-3
• Protein Family: Calponin
• UniProt Gene Name: CNN3
• UniProt Entry Name: CNN3_HUMAN

Uniprot Description

calponin 3: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton

Molecular Function: calmodulin binding; actin binding

Biological Process: epithelial cell differentiation; actomyosin structure organization and biogenesis