Rabbit Calnexin-CT Polyclonal Antibody | anti-CANX antibody

Calnexin-CT Antibody: PerCP

Cat. Num. AAA806691

• Gene Names: CANX; PP90
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: Calnexin-CT; Polyclonal Antibody; Calnexin-CT Antibody: PerCP; CANX; CNX; IP90; P90; anti-CANX antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 593

• Applicable Applications for anti-CANX antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: 1:2000 (WB)
• Conjugate: PerCP
• Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Rat tissue mix showing detection of Calnexin-CT protein using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Hair Follicles, Basal cells in epidermis, and second layer of epidermis.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear staining, cytoplasmic staining.)

Related Product Information for anti-CANX antibody

Background Info: Detects ~90kDa.

Scientific Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Product Categories/Family for anti-CANX antibody

Cell Signaling; Organelle Markers

References

1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97- 103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.

NCBI and Uniprot Product Information

• NCBI GI #: 50979014
• NCBI GeneID: 403908
• NCBI Accession #: NP_001003232.1
• NCBI GenBank Nucleotide #: NM_001003232.1
• UniProt Accession #: P24643; P79140
• Molecular Weight: 67,603 Da
• NCBI Official Full Name: calnexin
• NCBI Official Symbol: CANX
• NCBI Official Synonym Symbols: PP90
• NCBI Protein Information: calnexin
• UniProt Protein Name: Calnexin
• UniProt Gene Name: CANX
• UniProt Entry Name: CALX_CANFA

Uniprot Description

Calnexin: a calcium-binding protein of the calreticulin family. A type I membrane protein of the endoplasmic reticulum .Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. May act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Motility/polarity/chemotaxis; Calcium-binding