Rabbit anti-Human, Mouse Calcyclin Polyclonal Antibody | anti-CACY antibody

Calcyclin, CT (Protein S100-A6, S100 Calcium-binding Protein A6, S100A6, Prolactin Receptor-associated Protein, PRA, Growth Factor-inducible Protein 2A9, MLN 4, CACY) (MaxLight 750)

Cat. Num. AAA6466688

• Gene Names: S100A6; 2A9; PRA; 5B10; CABP; CACY; S10A6
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Calcyclin; Polyclonal Antibody; CT (Protein S100-A6; S100 Calcium-binding Protein A6; S100A6; Prolactin Receptor-associated Protein; PRA; Growth Factor-inducible Protein 2A9; MLN 4; CACY) (MaxLight 750); anti-CACY antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human S100A6. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-CACY antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human S100A6 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of S100A6 antibody (C-term) in mouse lung tissue lysates (35ug/lane). S100A6 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of S100A6 (arrow) using rabbit polyclonal S100A6 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the S100A6 gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(S100A6 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CACY antibody

Antibodies; Binding Proteins; Calcium

References

1. van Dieck, J. J. Biol. Chem. 284(20), 13804-13811 (2009). 2. Tsoporis, J.N., Izhar, S. J. Biol. Chem. 283(44), 30174-30183 (2008). 3. Lee, Y.T., Dimitrova, Y.N. Biochemistry 47(41), 10921-10932 (2008). 4. Melle, C., Ernst, G. PLoS ONE 3(12), E3767 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 7657532
• NCBI GeneID: 6277
• NCBI Accession #: NP_055439
• NCBI GenBank Nucleotide #: NM_014624.3
• UniProt Accession #: P06703; Q5RHS4; D3DV39
• Molecular Weight: 12.3 kDa (110aa) confirmed by MALDI-TOF
• NCBI Official Full Name: protein S100-A6
• NCBI Official Synonym Full Names: S100 calcium binding protein A6
• NCBI Official Symbol: S100A6
• NCBI Official Synonym Symbols: 2A9; PRA; 5B10; CABP; CACY; S10A6
• NCBI Protein Information: protein S100-A6
• UniProt Protein Name: Protein S100-A6
• Protein Family: Calcyclin-binding protein
• UniProt Gene Name: S100A6
• UniProt Synonym Gene Names: CACY; PRA
• UniProt Entry Name: S10A6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A6: May function as calcium sensor and contribute to cellular calcium signaling (Potential). May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: ruffle; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; ion transmembrane transporter activity; calcium ion binding; tropomyosin binding; calcium-dependent protein binding

Biological Process: positive regulation of fibroblast proliferation; axonogenesis; signal transduction