Rabbit anti-Human Calcineurin Polyclonal Antibody | anti-CALNA antibody

Calcineurin, NT (Serine/Threonine Protein Phosphatase 2B Catalytic Subunit, alpha Isoform, Calmodulin-dependent Calcineurin A Subunit, alpha Isoform, CAM-PRP Catalytic Subunit, PPP3CA, CALNA, CNA) (AP)

Cat. Num. AAA6466492

• Gene Names: PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Calcineurin; Polyclonal Antibody; NT (Serine/Threonine Protein Phosphatase 2B Catalytic Subunit; alpha Isoform; Calmodulin-dependent Calcineurin A Subunit; CAM-PRP Catalytic Subunit; PPP3CA; CALNA; CNA) (AP); anti-CALNA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PPP3CA.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CALNA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PPP3CA (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PPP3CA Pab in mouse kidney tissue lysate.PPP3CA(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CALNA antibody

Antibodies; Enzymes; Phosphatase

References

1. Rodriguez, A., et al., J. Biol. Chem. 280(11):9980-9984 (2005). 2. Shirane, M., et al., Nat. Cell Biol. 5(1):28-37 (2003). 3. Bengoechea-Alonso, M.T., et al., Nitric Oxide 8(1):65-74 (2003). 4. Huai, Q., et al., Proc. Natl. Acad. Sci. USA 99(19):12037-12042 (2002). 5. Bennasser, Y., et al., Virology 303(1):174-180 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 6715568
• NCBI GeneID: 5530
• NCBI Accession #: NP_000935
• NCBI GenBank Nucleotide #: NM_000944
• UniProt Accession #: Q08209; Q8TAW9; A1A441; A8K3B7; A8W6Z7; A8W6Z8; B5BUA2
• Molecular Weight: 51,245 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 3, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP3CA
• NCBI Official Synonym Symbols: CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; CAM-PRP catalytic subunit; calcineurin A alpha; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatas
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• Protein Family: Calcineurin
• UniProt Gene Name: PPP3CA
• UniProt Synonym Gene Names: CALNA; CNA
• UniProt Entry Name: PP2BA_HUMAN

Uniprot Description

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; Z disc; cytosol; calcineurin complex; sarcolemma

Molecular Function: calmodulin binding; protein dimerization activity; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; protein heterodimerization activity; protein serine/threonine phosphatase activity; drug binding; calcium ion binding

Biological Process: regulation of synaptic transmission; positive regulation of NFAT protein import into nucleus; T cell activation; response to amphetamine; skeletal muscle fiber development; protein amino acid dephosphorylation; negative regulation of insulin secretion; calcineurin-NFAT signaling pathway; transition between fast and slow fiber; dephosphorylation; protein import into nucleus; calcium ion transport; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; G1/S transition of mitotic cell cycle