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Western Blot (WB) (Western blot analysis of extracts from rat Brian, using CADM2 antibody. )

Rabbit CADM2 Polyclonal Antibody | anti-CADM2 antibody

CADM2 Antibody

Gene Names
CADM2; NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CADM2; Polyclonal Antibody; CADM2 Antibody; CADM2_HUMAN; Cell adhesion molecule 2; IgSF4D; Immunoglobulin superfamily member 4D; NECL 2; Necl 3; NECL-3; NECL2; Necl3; Nectin like protein 3; Nectin-like protein 3; synCAM 2; synCAM2; anti-CADM2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CADM2 antibody detects endogenous levels of total CADM2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
435
Applicable Applications for anti-CADM2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%), Xenopus (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from rat Brian, using CADM2 antibody. )

Western Blot (WB) (Western blot analysis of extracts from rat Brian, using CADM2 antibody. )

Immunofluorescene (IF)

(MBS9604993 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescene (IF) (MBS9604993 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using CADM2 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using CADM2 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2, mouse brain, using CADM2 Antibody.)

Western Blot (WB) (Western blot analysis of extracts from HepG2, mouse brain, using CADM2 Antibody.)
Related Product Information for anti-CADM2 antibody
Function: Adhesion molecule that engages in homo-and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes.
Similarity: Belongs to the nectin family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47 kDa
NCBI Official Full Name
cell adhesion molecule 2 isoform 1
NCBI Official Synonym Full Names
cell adhesion molecule 2
NCBI Official Symbol
CADM2
NCBI Official Synonym Symbols
NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2
NCBI Protein Information
cell adhesion molecule 2
UniProt Protein Name
Cell adhesion molecule 2
Protein Family
UniProt Gene Name
CADM2
UniProt Synonym Gene Names
IGSF4D; NECL3; IgSF4D; NECL-3; SynCAM 2

NCBI Description

This gene encodes a member of the synaptic cell adhesion molecule 1 (SynCAM) family which belongs to the immunoglobulin (Ig) superfamily. The encoded protein has three Ig-like domains and a cytosolic protein 4.1 binding site near the C-terminus. Proteins belonging to the protein 4.1 family crosslink spectrin and interact with other cytoskeletal proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Adhesion molecule that engages in homo- and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes.

Research Articles on CADM2

Similar Products

Product Notes

The CADM2 cadm2 (Catalog #AAA9604993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CADM2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CADM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CADM2 cadm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CADM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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