Rabbit anti-Human Cadherin-13 Polyclonal Antibody | anti-CDH13 antibody

Cadherin-13, NT (Cadherin, H, Truncated Cadherin, T-cadherin, T-cad, Heart Cadherin, H-cadherin, P105, CDH13, CDHH) (APC)

Cat. Num. AAA6466471

• Gene Names: ENTPD3; HB6; CD39L3; NTPDase-3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Cadherin-13; Polyclonal Antibody; NT (Cadherin; H; Truncated Cadherin; T-cadherin; T-cad; Heart Cadherin; H-cadherin; P105; CDH13; CDHH) (APC); anti-CDH13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDH13.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CDH13 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CDH13 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDH13 (arrow) using rabbit polyclonal CDH13 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH13 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with H Cadherin (CDH13) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using H Cadherin (CDH13) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CDH13 antibody

Antibodies; Cadherin

References

1. Qian, Z.R., Mod. Pathol. 20(12), 1269-1277 (2007). 2. Tsou, J.A., Mol. Cancer 6, 70 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 166197701
• NCBI GeneID: 956
• NCBI Accession #: NP_001248
• NCBI GenBank Nucleotide #: NM_001248.2
• UniProt Accession #: P55290; O60495; Q8N6K2; B2R8D0; G5E9N0; O75355
• Molecular Weight: Predicted: 78; 84 kDa; ; Observed: 85 kDa
• NCBI Official Full Name: Homo sapiens ectonucleoside triphosphate diphosphohydrolase 3 (ENTPD3), mRNA
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 3
• NCBI Official Symbol: ENTPD3
• NCBI Official Synonym Symbols: HB6; CD39L3; NTPDase-3
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 3; NTPDase 3; ecto-ATPase 3; ecto-ATPDase 3; ecto-apyrase 3; CD39 antigen-like 3; ecto-ATP diphosphohydrolase 3
• UniProt Protein Name: Cadherin-13
• Protein Family: Cadherin
• UniProt Gene Name: CDH13
• UniProt Synonym Gene Names: CDHH; H-cadherin; T-cad; T-cadherin
• UniProt Entry Name: CAD13_HUMAN

NCBI Description

This gene encodes a plasma membrane-bound divalent cation-dependent E-type nucleotidase. The encoded protein is involved in the regulation of extracellular levels of ATP by hydrolysis of it and other nucleotides. Multiple transcript variants have been described. [provided by RefSeq, May 2014]

Uniprot Description

CDH13: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 16q23.3

Cellular Component: extracellular space; neuron projection; focal adhesion; cytoplasm; plasma membrane; caveola; external side of plasma membrane

Molecular Function: adiponectin binding; protein homodimerization activity; cadherin binding; low-density lipoprotein binding; calcium ion binding

Biological Process: lamellipodium biogenesis; intercellular junction assembly and maintenance; positive regulation of positive chemotaxis; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; positive regulation of calcium-mediated signaling; negative regulation of cell adhesion; keratinocyte proliferation; Rho protein signal transduction; regulation of epidermal growth factor receptor signaling pathway; low density lipoprotein mediated signaling; negative regulation of cell proliferation; localization within membrane; calcium-dependent cell-cell adhesion; regulation of endocytosis; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; sprouting angiogenesis; positive regulation of cell-matrix adhesion; homophilic cell adhesion; positive regulation of cell migration