Rabbit anti-Human CAD Polyclonal Antibody | anti-CAD antibody

CAD (Phospho-Thr456) Antibody

Cat. Num. AAA9412380

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: CAD; Polyclonal Antibody; CAD (Phospho-Thr456) Antibody; PYR1; CAD protein; EC 2.1.3.2; EC 3.5.2.3; anti-CAD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of CAD only when phosphorylated at threonine 456.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2162

• Applicable Applications for anti-CAD antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD.
• Modification: Phospho-Thr456
• Target Name: CAD
• Preparation and Storage: Store at -20 degree C/1 year

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-CAD antibody

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.Iwahana H., Biochem. Biophys. Res. Commun. 219:249-255(1996).Davidson J.N., DNA Cell Biol. 9:667-676(1990).Olsen J.V., Cell 127:635-648(2006).

Product Categories/Family for anti-CAD antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 807045910
• NCBI GeneID: 790
• NCBI Accession #: NP_001293008.1
• NCBI GenBank Nucleotide #: NM_001306079.1
• UniProt Accession #: P27708; Q6P0Q0; Q96CK3; D6W552
• Molecular Weight: 242,984 Da
• NCBI Official Full Name: CAD protein isoform 2
• NCBI Official Synonym Full Names: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• NCBI Official Symbol: CAD
• NCBI Protein Information: CAD protein
• UniProt Protein Name: CAD protein
• Protein Family: Cadherin
• UniProt Gene Name: CAD
• UniProt Entry Name: PYR1_HUMAN

NCBI Description

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.

Protein type: Ligase; EC 3.5.2.3; Hydrolase; Amino Acid Metabolism - alanine, aspartate and glutamate; Nucleotide Metabolism - pyrimidine; EC 2.1.3.2; Transferase; EC 6.3.5.5

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: nucleoplasm; protein complex; cell projection; nuclear matrix; membrane; cell soma; plasma membrane; terminal button; cytosol; nucleus

Molecular Function: identical protein binding; carbamoyl-phosphate synthase (ammonia) activity; enzyme binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; zinc ion binding; aspartate carbamoyltransferase activity; ATP binding; protein kinase activity; dihydroorotase activity

Biological Process: lactation; organ regeneration; arginine biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; heart development; UTP biosynthetic process; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; glutamine metabolic process; female pregnancy; response to testosterone stimulus; liver development; response to caffeine; 'de novo' pyrimidine base biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; glutamine catabolic process; pyrimidine nucleoside biosynthetic process; drug metabolic process; response to amine stimulus; urea cycle

Disease: Congenital Disorder Of Glycosylation, Type Iz