Rabbit anti-Human CACNG4 Polyclonal Antibody | anti-CACNG4 antibody

CACNG4, ID (CACNG4, Voltage-dependent calcium channel gamma-4 subunit, Neuronal voltage-gated calcium channel gamma-4 subunit, Transmembrane AMPAR regulatory protein gamma-4) (Biotin)

Cat. Num. AAA6280399

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CACNG4; Polyclonal Antibody; ID (CACNG4; Voltage-dependent calcium channel gamma-4 subunit; Neuronal voltage-gated calcium channel gamma-4 subunit; Transmembrane AMPAR regulatory protein gamma-4) (Biotin); Transmembrane AMPAR regulatory protein gamma-4; anti-CACNG4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CACNG4 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CACNG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the Central region of human CACNG4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CACNG4 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CACNG4 antibody detected the CACNG4 protein (arrow).)

Flow Cytometry (FC/FACS)

(CACNG4 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CACNG4 antibody

The protein encoded by this gene is a type I transmembrane AMPA receptor regulatory protein (TARP). TARPs regulate both trafficking and channel gating of the AMPA receptors. This gene is part of a functionally diverse eight-member protein subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two family members, a type II TARP and a calcium channel gamma subunit.

Product Categories/Family for anti-CACNG4 antibody

Antibodies; Transmembrane Proteins

References

Sager, C., et al. Neuroscience 158(1):45-54(2009). Chen, R.S., et al. Cell Biochem. Biophys. 47(2):178-186(2007). Moss, F.J., et al. BMC Neurosci 4, 23 (2003): Chu, P.J., et al. Gene 280 (1-2), 37-48 (2001): Burgess, D.L., et al. Genomics 71(3):339-350(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7656948
• NCBI GeneID: 27092
• NCBI Accession #: NP_055220.1
• NCBI GenBank Nucleotide #: NM_014405.3
• UniProt Accession #: Q9UBN1; B2RCK0
• Molecular Weight: 36,579 Da
• NCBI Official Full Name: voltage-dependent calcium channel gamma-4 subunit
• NCBI Official Synonym Full Names: calcium channel, voltage-dependent, gamma subunit 4
• NCBI Official Symbol: CACNG4
• NCBI Protein Information: voltage-dependent calcium channel gamma-4 subunit; TARP gamma-4; transmembrane AMPAR regulatory protein gamma-4; neuronal voltage-gated calcium channel gamma-4 subunit
• UniProt Protein Name: Voltage-dependent calcium channel gamma-4 subunit
• Protein Family: Voltage-dependent calcium channel
• UniProt Gene Name: CACNG4
• UniProt Synonym Gene Names: TARP gamma-4
• UniProt Entry Name: CCG4_HUMAN

NCBI Description

The protein encoded by this gene is a type I transmembrane AMPA receptor regulatory protein (TARP). TARPs regulate both trafficking and channel gating of the AMPA receptors. This gene is part of a functionally diverse eight-member protein subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two family members, a type II TARP and a calcium channel gamma subunit. [provided by RefSeq, Dec 2010]

Uniprot Description

Function: Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. Ref.6

Subunit structure: The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs). Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG5, CACNG7 and CACNG8

By similarity.

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity.

Sequence similarities: Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.