Rabbit anti-Human CABP4 Polyclonal Antibody | anti-CABP4 antibody

CABP4, CT (CABP4, Calcium-binding protein 4) (Biotin)

Cat. Num. AAA6280289

• Gene Names: CABP4; CSNB2B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CABP4; Polyclonal Antibody; CT (CABP4; Calcium-binding protein 4) (Biotin); Calcium-binding protein 4; anti-CABP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CABP4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CABP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the C-terminal region of human CABP4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CABP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CABP4 antibody detected the CABP4 protein (arrow).)

Related Product Information for anti-CABP4 antibody

This gene encodes a member of the CABP family of calcium binding protein characterized by four EF-hand motifs. Mutations in this gene are associated with congenital stationary night blindness type 2B.

Product Categories/Family for anti-CABP4 antibody

Antibodies; Binding Proteins; Calcium

References

Aldahmesh, M.A., et al. Mol. Vis. 16, 207-212 (2010): Littink, K.W., et al. Invest. Ophthalmol. Vis. Sci. 50(5):2344-2350(2009). Zeitz, C., et al. Am. J. Hum. Genet. 79(4):657-667(2006). Taylor, T.D., et al. Nature 440(7083):497-500(2006). Haeseleer, F., et al. Nat. Neurosci. 7(10):1079-1087(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21624641
• NCBI GeneID: 57010
• NCBI Accession #: NP_660201.1
• NCBI GenBank Nucleotide #: NM_145200.3
• UniProt Accession #: P57796; Q8N4Z2; Q8WWY5
• Molecular Weight: 30,433 Da
• NCBI Official Full Name: calcium-binding protein 4
• NCBI Official Synonym Full Names: calcium binding protein 4
• NCBI Official Symbol: CABP4
• NCBI Official Synonym Symbols: CSNB2B
• NCBI Protein Information: calcium-binding protein 4
• UniProt Protein Name: Calcium-binding protein 4
• Protein Family: Calcium-binding protein
• UniProt Gene Name: CABP4
• UniProt Synonym Gene Names: CaBP4
• UniProt Entry Name: CABP4_HUMAN

NCBI Description

This gene encodes a member of the CABP family of calcium binding protein characterized by four EF-hand motifs. Mutations in this gene are associated with congenital stationary night blindness type 2B. [provided by RefSeq, Mar 2010]

Uniprot Description

Function: Involved in normal synaptic function through regulation of Ca2+ influx and neurotransmitter release in photoreceptor synaptic terminals and in auditory transmission. Modulator of CACNA1D and CACNA1F, suppressing the calcium-dependent inactivation and shifting the activation range to more hyperpolarized voltages

By similarity.

Subunit structure: Interacts with CACNA1F and CACNA1D (via IQ domain) in a calcium independent manner

By similarity. Interacts (via N-terminus) with UNC119

By similarity.

Subcellular location: Cytoplasm. Note: Found in rod spherules and cone pedicles of the presynapses from both types of photoreceptors

By similarity. Ref.5

Tissue specificity: Expressed in retina and in the inner hair cells (IHC) of the cochlea.

Post-translational modification: Phosphorylated. Phosphorylation levels change with the light conditions and regulate the activity

By similarity.

Involvement in disease: Night blindness, congenital stationary, 2B (CSNB2B) [MIM:610427]: A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.6

Sequence similarities: Contains 4 EF-hand domains.