Rabbit anti-Human CABP1 Polyclonal Antibody | anti-CABP1 antibody

CABP1, CT (Calcium-binding Protein 1, CaBP1, Caldendrin, Calbrain) (FITC)

Cat. Num. AAA6466308

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CABP1; Polyclonal Antibody; CT (Calcium-binding Protein 1; CaBP1; Caldendrin; Calbrain) (FITC); anti-CABP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CABP1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 227

• Applicable Applications for anti-CABP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CABP1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of anti-CABP1 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane).CABP1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CABP1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(CABP1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CABP1 antibody detected the CABP1 protein (arrow).)

Product Categories/Family for anti-CABP1 antibody

Antibodies; Binding Proteins; Calcium

References

1. Haynes, L.P., Proteomics 6(6), 1822-1832 (2006). 2. Wingard, J.N., J. Biol. Chem. 280(45), 37461-37470 (2005). 3. Zhou, H., J. Biol. Chem. 280(33), 29612-29619 (2005). 4. Haeseleer, F., J. Biol. Chem. 275(2), 1247-1260 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 13929432
• NCBI Accession #: NP_112482
• NCBI GenBank Nucleotide #: NP_112482.1
• UniProt Accession #: Q9NZU7
• Molecular Weight: 25.979
• NCBI Official Full Name: calcium-binding protein 1 isoform 1
• UniProt Protein Name: Calcium-binding protein 1
• Protein Family: Calcium-binding protein
• UniProt Gene Name: CABP1
• UniProt Entry Name: CABP1_HUMAN

Uniprot Description

CABP1: Modulates calcium-dependent activity of inositol 1,4,5- triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D. Inhibits TRPC5 channels. Prevents NMDA receptor- induced cellular degeneration. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Calcium-binding; Inhibitor

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space; internal side of plasma membrane; protein complex; dendrite; postsynaptic density; cell cortex; cytosol; Golgi membrane; postsynaptic membrane; cytoskeleton; cell soma; perinuclear region of cytoplasm; plasma membrane; cell junction; nucleus

Molecular Function: enzyme inhibitor activity; protein domain specific binding; protein binding; protein homodimerization activity; nuclear localization sequence binding; type 3 metabotropic glutamate receptor binding; calcium ion binding; calcium-dependent protein binding

Biological Process: negative regulation of catalytic activity; negative regulation of protein import into nucleus