Rabbit anti-Human, Mouse CA3 Polyclonal Antibody | anti-CA3 antibody

CA3, NT (Carbonic Anhydrase 3, Carbonic Anhydrase III, CA-III, Carbonate Dehydratase III) (MaxLight 650)

Cat. Num. AAA6467006

• Gene Names: CA3; Car3; CAIII
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CA3; Polyclonal Antibody; NT (Carbonic Anhydrase 3; Carbonic Anhydrase III; CA-III; Carbonate Dehydratase III) (MaxLight 650); anti-CA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CA3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
• Sequence Length: 260

• Applicable Applications for anti-CA3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CA3 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CA3 antibody (N-term) in HepG2 cell line lysates (35ug/lane). CA3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CA3 antibody (N-term) in mouse kidney tissue lysates (35ug/lane). CA3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CA3 antibody

Antibodies; Enzymes

References

1. Du, A.L., Autoimmunity 42(3), 209-215 (2009). 2. Dai, H.Y., Mol. Carcinog. 47(12), 956-963 (2008). 3. Gailly, P., Kidney Int. 74(1), 52-61 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4885099
• NCBI GeneID: 761
• NCBI Accession #: NP_005172
• NCBI GenBank Nucleotide #: NP_005172.1
• UniProt Accession #: P07451
• Molecular Weight: 29.557
• NCBI Official Full Name: carbonic anhydrase 3
• NCBI Official Synonym Full Names: carbonic anhydrase 3
• NCBI Official Symbol: CA3
• NCBI Official Synonym Symbols: Car3; CAIII
• NCBI Protein Information: carbonic anhydrase 3
• UniProt Protein Name: Carbonic anhydrase 3
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA3
• UniProt Synonym Gene Names: CA-III
• UniProt Entry Name: CAH3_HUMAN

NCBI Description

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]

Uniprot Description

CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding; nickel ion binding

Biological Process: response to ethanol; bicarbonate transport; one-carbon compound metabolic process; response to oxidative stress