Rabbit anti-Human CA2 / Carbonic Anhydrase II Polyclonal Antibody | anti-CA2 antibody

Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II

Cat. Num. AAA240961

• Gene Names: CA2; CAC; CAII; Car2; CA-II; HEL-76
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Ion exchange Chromatography
• Synonyms: CA2 / Carbonic Anhydrase II; Polyclonal Antibody; Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II; Anti-CA2 / Carbonic Anhydrase II Antibody IHC-plus; CA2; CA-II; CAII; CAC; Car2; Carbonate dehydratase II; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase; Carbonic anhydrase 2; Carbonic anhydrase B; Human CA2; Carbonic Anhydrase II; anti-CA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Carbonic Anhydrase II [Human Erythrocytes].
• Purity/Purification: Ion exchange Chromatography
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for anti-CA2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Resuspension Liquid Description: After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-Carbonic Anhydrase II antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-CA2 antibody

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4557395
• NCBI GeneID: 760
• NCBI Accession #: NP_000058.1
• NCBI GenBank Nucleotide #: NM_000067.2
• UniProt Accession #: P00918; Q6FI12; Q96ET9; B2R7G8
• Molecular Weight: 29,246 Da
• NCBI Official Full Name: carbonic anhydrase 2 isoform 1
• NCBI Official Synonym Full Names: carbonic anhydrase II
• NCBI Official Symbol: CA2
• NCBI Official Synonym Symbols: CAC; CAII; Car2; CA-II; HEL-76
• NCBI Protein Information: carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; epididymis luminal protein 76
• UniProt Protein Name: Carbonic anhydrase 2
• Protein Family: Beta-carotene hydroxylase
• UniProt Gene Name: CA2
• UniProt Synonym Gene Names: CAC; CA-II
• UniProt Entry Name: CAH2_HUMAN

NCBI Description

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Lyase; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; basolateral plasma membrane; axon; apical part of cell; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function: carbonate dehydratase activity; protein binding; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to estrogen stimulus; response to zinc ion; bicarbonate transport; regulation of intracellular pH; morphogenesis of an epithelium; positive regulation of bone resorption; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3