Western Blot (WB)
((LEFT)Western blot analysis of C7orf47 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane).C7orf47 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of C7orf47 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane).C7orf47 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse C7orf47 Polyclonal Antibody | anti-PPP1R35 antibody
C7orf47, NT (PPP1R35, C7orf47, Protein phosphatase 1 regulatory subunit 35)
Gene Names
PPP1R35; C7orf47
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
C7orf47; Polyclonal Antibody; NT (PPP1R35; Protein phosphatase 1 regulatory subunit 35); Anti -C7orf47; anti-PPP1R35 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-PPP1R35 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunogen
C7orf47 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human C7orf47.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
((LEFT)Western blot analysis of C7orf47 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane).C7orf47 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of C7orf47 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane).C7orf47 (arrow) was detected using the purified Pab.)
Western Blot (WB)
((LEFT)Western blot analysis of C7orf47 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane).C7orf47 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of C7orf47 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane).C7orf47 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(C7orf47 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C7orf47 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(C7orf47 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C7orf47 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-PPP1R35 antibody
The function of this protein has not been specifically defined.
Product Categories/Family for anti-PPP1R35 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,953 Da
NCBI Official Full Name
Chromosome 7 open reading frame 47
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 35
NCBI Official Symbol
PPP1R35
NCBI Official Synonym Symbols
C7orf47
NCBI Protein Information
protein phosphatase 1 regulatory subunit 35; UPF0683 protein C7orf47
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 35
Protein Family
UniProt Gene Name
PPP1R35
UniProt Synonym Gene Names
C7orf47
UniProt Entry Name
PPR35_HUMAN
Uniprot Description
MGC22793: Inhibits PPP1CA phosphatase activity. Belongs to the PPP1R35 family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 7q22.1
Molecular Function: protein phosphatase inhibitor activity; phosphatase binding
Similar Products
Product Notes
The PPP1R35 ppp1r35 (Catalog #AAA6002929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C7orf47, NT (PPP1R35, C7orf47, Protein phosphatase 1 regulatory subunit 35) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C7orf47 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the PPP1R35 ppp1r35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C7orf47, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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