Immunohistochemistry (IHC)
Rabbit anti-Mouse, Rat C6orf150 Polyclonal Antibody | anti-Mb21d1 antibody
C6orf150 Antibody
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:500
Immuohistochemistry: 1:150
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Catalyzes the formation of cyclic GMP-AMP from ATP and GTP. Has antiviral activity through acting as a cystolic DNA senser.
The cyclic guanosine monophosphate-adenosine monophosphate (GMP-AMP) synthase (cGAS) belongs to the nucleotidyl transferase family, catalyzes the formation of cGMP-AMP (cGAMP) from ATP and GTP. The presence of cytosolic DNA in the cytoplasm of mammalian cells is a danger signal that triggers the most immune responses which produces the type I interferons. The DNA signaling pathway requires an adapter protein STING and transcription factor IRF3. The mechanism through which interferons are induced by cytosolic DNA involves cyclic GMP-AMP synthase, which binds and activates the endoplasmic reticulum adapter protein STING (also known as MITA, MPYS, or ERIS) that activates the transcription activity of interferon syntheses. The STING recruits and activates the cytosolic kinase IKK and TBK1, which activates the transcription factor NF-kB and transcription factor IRF3. The NF-kB and IRF3 enters the nucleus and in concert induce interferons and other cytokines (1). The DNA-dependent RNA polymerase III also acts as a sensor and synthesize AT-rich DNA such as poly (dA:dT) into n RNA ligand that stimulates RIG-l pathway to induce interferons (2, 3).
Most DNA does not activate DNA-dependent RNA polymerase and downstream RIGI pathways, but instead activates STING pathway via cGAS and cGAMP stimulation for interferon induction. DNA can be introduced by viral or bacterial infection, or leakage form nucleus or mitochondria under some pathological conditions that cause auto immune disease like Lupus. Cytosolic cGMP-AMP synthesized in response to DNA by cGAS acts as a second messenger in innate immune Signaling. cGAS gene encodes for a 522 aa protein that has a Mab-21 motif commonly found in C. elegans that is required for choice of alternate cell fates in male tail cells.
Cyclic-GAS-selective antibodies were generated against peptides taken from human cGAS protein. The cGAS-selective antibodies were affinity purified on an immobilized antigen based affinity matrices. The isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti-cGAS-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. Western blot positive control samples in "ready-to-use" SDS-PAGE sample buffer (PC-CGAS) and antigenic blocking peptide (P-CGAS. l ) for cGAS are available. All antibodies can be conjugated to fluorophores and other secondary enzymes as an additional service. MyBioSource provides custom antibody production services for researchers that are looking for high affinity monoclonal and polyclonal antibodies in various host animal species.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
MB21D1: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
Protein type: Enzyme, cellular metabolism; EC 2.7.7.86
Cellular Component: cytoplasm; cytosol
Molecular Function: ATP binding; DNA binding; GTP binding; metal ion binding; nucleotide binding; nucleotidyltransferase activity; transferase activity
Biological Process: activation of innate immune response; cyclic nucleotide biosynthetic process; defense response to virus; immune system process; innate immune response; positive regulation of defense response to virus by host
Research Articles on Mb21d1
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Product Notes
The Mb21d1 mb21d1 (Catalog #AAA540138) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C6orf150 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C6orf150 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:150 Western Blot: 1:500 Immuohistochemistry: 1:150. Researchers should empirically determine the suitability of the Mb21d1 mb21d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C6orf150, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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