Rabbit anti-Human C6 Polyclonal Antibody | anti-C6 antibody

C6, NT (Complement Component C6) (APC)

Cat. Num. AAA6469970

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: C6; Polyclonal Antibody; NT (Complement Component C6) (APC); anti-C6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C6.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-C6 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 30-58 in the N-terminal region of human C6.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MDA-MB231 cell line lysates (35ug/lane) using C7850-35G. C6 (arrow) was detected using C7850-35G.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma reacted with C7850-35G, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of C7850-35G for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MDA-231 cells using C7850-35G (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with MDA-MB231 cells using C7850-35G followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-C6 antibody

Antibodies; Complement

References

1. Wu, C., et.al., Proteomics 7(11), 1775-1785 (2007).1. Wu, C., et.al., Proteomics 7(11), 1775-1785 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 169636415
• NCBI GeneID: 729
• NCBI Accession #: NP_000056.2
• NCBI GenBank Nucleotide #: NM_000065.3
• UniProt Accession #: P13671
• Molecular Weight: 105 KD
• NCBI Official Full Name: complement component C6
• NCBI Official Synonym Full Names: complement component 6
• NCBI Official Symbol: C6
• NCBI Protein Information: complement component C6
• UniProt Protein Name: Complement component C6
• Protein Family: Complement component
• UniProt Gene Name: C6
• UniProt Entry Name: CO6_HUMAN

NCBI Description

This gene encodes a component of the complement cascade. The encoded protein is part of the membrane attack complex that can be incorporated into the cell membrane and cause cell lysis. Mutations in this gene are associated with complement component-6 deficiency. Transcript variants encoding the same protein have been described.[provided by RefSeq, Nov 2012]

Uniprot Description

C6: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C6 are the cause of complement component 6 deficiency (C6D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane attack complex; extracellular region

Molecular Function: protein binding

Biological Process: positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; in utero embryonic development; cytolysis; regulation of complement activation; innate immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 6 Deficiency